九香止泻颗粒对致泻性海洋弧菌影响及其防治急性腹泻相关作用机理的研究-中药学专业论文.docxVIP

中文摘要目的：探讨九香止泻颗粒对致泻性海洋弧菌、新斯的明引起的小鼠小 中文摘要 目的：探讨九香止泻颗粒对致泻性海洋弧菌、新斯的明引起的小鼠小 肠功能亢进、番泻叶所致小鼠急性腹泻模型及小鼠腹腔毛细血管通透 性影响。 方法：采用琼脂扩散法测定抑菌圈直径，液体培基测定法与平皿倾注 法测定最低抑菌浓度(MIC)和最低杀菌浓度(MBC)；皮下注射甲硫酸 新斯的明造模，灌胃炭末生理盐水，测定小肠推进率；番泻叶造模， 比较腹泻指数；尾静脉注射伊文思兰，腹腔注射醋酸造模，测定吸光 度。 结果：九香止泻颗粒组具有显著的抑制副溶血弧茵作用，其作用效果 优于肠康片(尸O．01)。九香止泻颗粒各剂量组小肠推进率显著降低 (尸0．05)；其中、高剂量组明显优于肠康组(P0．05)。九香止 泻颗粒各剂量组腹泻指数均显著降低(P0．05)，其作用效果优于肠 康组和颠茄磺苄啶组(尸0．05)。各剂量组吸光度明显降低(尸0．01)； 中剂量组明显优于肠康组(尸0．05)。 结论：结果提示九香止泻颗粒具有显著的抗致泻性海洋弧茵活性、抑 制小肠功能亢进、止泻和抗炎的作用；且相关作用优于肠康片和颠茄 磺苄啶片。 关键词：九香止泻颗粒海洋弧菌小肠推进腹泻指数通透性作用机 理 Research Research of the Effect of JiuXiangZhiXie Granule(Jxzxc)on Diarrhea Genic Marine Vibrio and the Mechanism of Prevention and Cure on Diarrhea Abs仃act Postgraduate：Yin Jin Tutor：Professor Peng Zhipei， Professor Teng Jiuxiang (TCM Univ．of Hunan，Changsha 410007，China) 【Object ire]To explore the effect ofJiuXiangZhiXie Granule(JXZXG) on diarrhea genic marine vibrio，the hyperintestine caused by Neostigmine，the acute diarrhea model caused by senna and the permeability of celiac capillary vessel． 【Method】Disk diffusion method was used to measure the diameter of the inhibition zone．And the minimum drug concentration(MIC)and the minimum bacterium concentration(MBC)were measured with agar dilution allergic method．Neostigmine was hypodermic injected to set models．Then 0．9％saline with charcoal powders was given in intragastric administration．The propellant rates of charcoal powder in small intestine were measured．Senna decoction was given to set models， and the diarrhea incidences were measured．Evans blue(EB)physiology saline was injected in tail vein．Then acetic acid Was injected in celiac．the absorbance in spectrophotometer were measured． 【Result】 JXZXG groups could inhibit Vibrio parahaemolyticus remarkably．And the effect was superior to the Changkang Tablet(CKT) group伊O．0 1)．the propellant rate of small intestine in each treatment groups were decreased obviously(P0．05)；The effect of middling and hi曲一dose groups was superior to CKT group(PO．05)．The