被动应力训练对兔冈上肌腱急性断裂术后修复过程-南方医科大学学报.PDFVIP

窑1020窑 南方医科大学学报(JSouthMedUniv) 2010;30(5) 被动应力训练对兔冈上肌腱急性断裂术后修复过程中碱性成纤维细胞生 长因子表达的影响 李 森袁闵少雄袁张 辉袁付国建袁王鹏程袁靳安民(南方医科大学珠江医院骨科中心袁广东广州510282) 摘要院目的通过研究应力刺激对其腱-骨修复碱性成纤维细胞生长因子渊b-FGF冤表达的影响袁探讨应力刺激在肩袖损伤 术后腱-骨修复中的作用遥 方法 选取 18只成年雄性新西兰白兔袁术前随机选取两只白兔处死袁以熟悉解剖结构及对照 用遥 余 16只白兔随机分为2组院CPM组渊 组与非CPM组渊B组冤遥 组术后第2周开始训练曰B组正常笼养遥 分别于 术后2尧4尧6尧8周训练结束后处死袁取材,每次每组各处死两只袁行b-FGF 细胞因子表达检测遥 结果 术后2周袁两组染色 均为阳性袁 组颜色较B组稍深袁面积稍显广泛曰术后4周袁 组染色颜色较B组明显加深袁面积明显广泛遥 组大量阳 性表达的成纤维细胞沿腱外膜平行排列曰术后6周袁两组染色阳性袁 颜色深度较前无明显变化袁B组较前变浅曰术后8 周袁两组染色仍为阳性袁颜色深度较前均变浅袁B组变浅较明显遥结论 被动应力训练渊CPM冤能够促进兔冈上肌腱急性断 裂术后腱-骨界面修复早期b-FGF表达袁从而增进芋型胶原合成袁加快肩袖损伤术后早期腱-骨修复进程遥 关键词院兔曰冈上肌腱曰修复曰持续被动训练曰碱性成纤维细胞生长因子 中图分类号院R687 文献标识码院 文章编号院673-4254渊2010冤05-1020-04 Effect of continuous passive motion on basic fibroblast growth factor expression during tendon-bonerepairaftersurgicalrepairofacuteruptureofthesupraspinatustendoninrabbits LISen,MINShao-xiong,ZHANGHui,FUGuo-jian,WANGPeng-cheng,JINAn-min DepartmentofOrthopedics,ZhujiangHospital,SouthernMedicalUniversity,Guangzhou510282,China Abstract: ObjectiveTostudytheeffectofcontinuouspassivemotion (CPM) onbasicfibroblastgrowthfactor (b-FGF) expressionduringtendon-bonerepairinrabbitsandexploretheroleofstressinthepostoperativerepairafteracuterotatorcuff injury. Methods SixteenrabbitsrandomizedintoCPMgroup(n=8) andnon-CPMgroup(n=8) weresubjectedtosurgically inducedacuteruptureofthesupraspinatustendonandsubsequentsurgicalrepair, withanothertworabbitsservingasthe control.Twoweeksaftertheoperation,therabbitsinCPMgroupunderwentCPMtraining,andthoseinnon-CPMgroupwere normallyfedonly.At2,4,6,and8weeksaftertheoperation,2rabbitsfromeachgroupweresacrificedandthetissuesamples wereobtainedfordetectingthechangesinb-FGFexpression. ResultsTwoweeksaftertheoperation, b-FGFexpressionwas detectedinbothgroups, andtheCPMgroupshowedslightlyhigherandmorediffusiveexpr