鸡蛋膜荧光传感器光谱信息表征及三聚氰胺检测方法的构建-食品科学.PDF

328 2018, Vol.39, No.22 食品科学 ※安全检测 鸡蛋膜荧光传感器光谱信息表征及三聚氰胺 检测方法的构建 张 沛，李 莹，王增利，王世平* （中国农业大学食品科学与营养工程学院，北京 100083 ） 摘 要：为了实现奶制品中三聚氰胺的快速准确检测，基于溶液中三聚氰胺能使荧光素的荧光强度在一定浓度范围 内线性增强的特点，利用天然食品分析物鸡蛋膜作为生物膜基质，建立一种固定荧光素到鸡蛋膜上的生物传感器并 用于三聚氰胺检测的方法，优化传感器体系中的荧光素浓度及戊二醛质量分数。该鸡蛋膜传感器的线性工作范围为 1～500 µg/L ，检出限为0.47 µg/L 。相比溶液中三聚氰胺的检测方法鸡蛋膜固相传感器检测方法特异性强，稳定性 好，具有更高的选择性和低检出限。该种鸡蛋膜荧光传感器能成功应用于市售奶制品中三聚氰胺的检测。 关键词：鸡蛋膜；荧光素；三聚氰胺；传感器 Spectral Characterization of Fluorescent Sensor Immobilized with Eggshell Membrane and Method Development for Melamine Detection * ZHANG Pei, LI Ying, WANG Zengli, WANG Shiping (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) Abstract: This study aimed to develop a method for rapid and precise detection of melamine in milk products. Based on the phenomenon that the melamine can linearly increase the fluorescence intensity of fluorescein within a certain concentration range, we constructed an eggshell membrane biosensor with fluorescein immobilized on its surface to detect melamine. Fluorescein and glutaraldehyde concentration were optimized. The eggshell membrane sensor had a linear working range of 1–500 µg/L, and the limit of detection (LOD) for melamine was 0.47 µg/L. Compared to the methods used for the detection of melamine in aqueous solution, the eggshell membrane sensor was more specific and stable with higher selectivity and lower LOD. This sensor can be successfully applied to the detection of melamine in commercial milk products. Keywords: egg membrane; fluorescein;