超声波对试验性兔膝骨关节炎软骨细胞基质金属-中国康复医学杂志.PDFVIP

2011年，第26卷，第10期 ·基础研究· 超声波对实验性兔膝骨关节炎软骨细胞基质金属 蛋白酶-1表达的影响 李卫平 蹇 睿 胥方元1 1 1,2 摘要 目的：探讨超声波治疗对兔膝骨关节炎（KOA）模型软骨细胞基质金属蛋白酶-1（MMP-1）表达的影响。 方法：30只新西兰大白兔随机分为正常对照组、模型组和超声波治疗组，每组10只。将模型组和超声波治疗组的兔 左膝于伸直位石膏固定6周制作KOA模型。造模后超声波治疗组实施超声波治疗2周。8周后取各组软骨观察膝 软骨病理学形态改变、MMP-1表达的变化。 结果：采用Mankin评分比较病理学形态改变，正常对照组与模型组、模型组与超声波治疗组间比较，差异均有显著 性意义(P0.01)。软骨免疫组化MMP-1测定，正常对照组与模型组、模型组与超声波治疗组间比较，差异均有显著 性意义(P0.01)。 结论：超声波能有效治疗膝骨性关节炎，其作用机制可能与减少软骨细胞MMP-1的含量而利于软骨细胞的修复有 关。 关键词 骨关节炎；软骨细胞；超声波；基质金属蛋白酶-1 中图分类号：R493,R684 文献标识码：A 文章编号：1001-1242（2011）-10-0911-04 The effect of ultrasonic therapy on expression of matrix-metalloproteinase-1 in cartilage cells of rabbit with knee osteoarthritis/LI Weiping, JIAN Rui, XU Fangyuan//Chinese Journal of Rehabilitation Medi⁃ cine, 2011, 26(10): 911—914 Abstract Objective: To study the effect of ultrasound therapy on expression of matrix-metalloproteinase-1(MMP-1) in carti- lagecellsofrabbitwith knee osteoarthritis(KOA). Method: Thirty healthy New Zealand white rabbits were randomly divided into 3 groups: normal control group contained 10 rabbits, not modeling; the other 20 rabbits were randomly divided into model group and ultrasonic experimental group, being created osteoarthritis. The rabbits left knees were fixed within plaster cast in stretch position for 6 weeks to created KOA models. Then ultrasonic, experimental group was given ultrasonic treatment for 2 weeks. After 8 weeks cartilage changes on pathological morphology and expression of MMP-1 were observed inallgroups. Result: By Mankins score the severity of pathological lesion in model group was higher than that in normal con- trol group and u