医学毕业淋球菌不同耐药水平中mtrf基因的表达.docxVIP

XX大学 毕业论文 淋球菌不同耐药水平中mtrF基因的表达 姓 名： 2014年6月25日 作者：王冬梅，夏忠弟，邹明祥，齐素文 【摘要】 目的 探讨mtrF基因在多重耐药淋球菌中的表达及其与高水平多重 耐药淋球菌的关系。方法 ①纸片扩散法检测58株淋球菌对5种抗生素的敏感性； ②试管稀释法测定淋球菌对红霉素的最小抑菌浓度(MIC)；③采用RT PCR技 术测定敏感组、低 中等水平多重耐药组及高水平多重耐药组中mtrD mtrF基 因的表达。结果①58株淋球菌中对2种或者2种以上抗生素耐药的有30株， 占待测淋球菌的51.7%；②红霉素MIC为敏感、中介、耐药，分别为7株、21 株和30株，有17株MIC值32.0pg/mL；③高水平多重耐药组mtrF基因表达量 升高，与低 中等水平耐药组和敏感组比较，差异均有统计学意义(P0.01)；但后 两者之间无明显差界。耐药组mtrD基因表达量升高，与敏感组比较，差界均有 统计学意义(P0.01)；但两个耐药组之间无明显差异。结论mtrF基因在高水平多 重耐药组屮表达均高于敏感组和低 屮等水平多重耐药组，表明其在介导淋球菌 产生高水平多重耐药过程中可能发挥着十分重要的作用。 【关键词】 淋病奈瑟菌；多重耐药；mtrF MtrF gene expression in Neisseria gonorrhoeae of diflerent resistance levels ABSTRACT: Objective To study the relationship between the expression of mtrF gene and high level multiple resistant Neisseria gonorrhoea? Methods ① The susceptibility of 58 clinical Neisseria gonorrhoeae to 5 kinds of antibiotic agents was tested by disc diffusion method? ② The minimum inhibitory concentration (MIC) of erythromycin was determined by tube dilution method. (3) The expression of mtrD and mtrF gene in susceptive group, inter mediated resistant group and high level multiple resistant group was detected by semi quantitative RT PCR. Results ① There were 30 strains presenting resistance to two or more than two antimicrobial agents, which accounted for 51.7% of the 58 clinical strains.② The number of strains sensitive, intermediate and resistant to erythromycin was 7, 21 and 30, respectively, and there were 17 strains with erythromycin MIC32.0|ig /mL. ③ Compared with that in susceptive group and inter mediated resistant group, mtrF expression was up regulated in high level multiple resistant group (P0.01). The mtrF expression had no significant change in susceptive group or inter mediated resistant group. Compared with that in susceptive group, mtrD expression was up regulated in two resistant groups (P0.01). The mtrD expression had no significant change in high level multiple resistant group or inter mediated resistant group. Conclusion The mtrF expres