银杏花粉粗多糖脱色工艺研究.pdfVIP

136 2009, Vol. 30, No. 14 食品科学 ※工艺技术 郝功元，吴彩娥* ，杨剑婷，贾韶千，徐文斌 (南京林业大学森林资源与环境学院，江苏南京 210037) 为优选银杏花粉粗多糖的脱色材料，以多糖保留率和脱色率为考察指标，通过比较聚酰胺、树脂、粉 末活性炭和颗粒活性炭四种不同的脱色剂的脱色效果，选择一种较好的脱色剂进行单因素和正交试验。结果表明： 颗粒活性炭优于其他3 种脱色剂，通过正交试验法研究脱色条件，颗粒活性炭脱色最佳工艺参数为脱色时间4h 、脱 色温度50 ℃、脱色剂用量0.15g/ml 。 银杏花粉；脱色；颗粒活性炭 Decolorization of Crude Polysaccharide from Ginkgo biloba Pollen HAO Gong-yuan ，WU Cai-e* ，YANG Jian-ting ，JIA Shao-qian ，XU Wen-bin (College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China) Abstract ：Polyamides, D101 macroporous resin, powdered activated carbon and granular activated carbon were separately adopted to decolorize the crude polysaccharide from Ginkgo biloba pollen. Granular activated carbon had the best decolorization efficiency in terms of weighed score for retention rate of polysaccharide and decolorization rate. One-factor-at-a-time method was adopted to investigate the effects of three crucial factors (namely, decolorization duration, temperature and decolorant quantity) on decolorization of the crude polysaccharide. Subsequently, the three factors were optimized using orthogonal array design. The optimal decolorization duration, temperature and decolorant quantity were 50 ℃, 4 h and 0.15 g/ml, respectively. Under these conditions, the decolorization rate was over 72.84% and 88.19% of polysaccharide was recovered. Key words ：Ginkgo biloba pollen ；decolorization ；granular activated carbon 中图分类号：S789.9 文献标识码：A 文章编号：1002-6630(2009)14-0136-04 银杏又名公孙树、白果树，是最古老的中生代孑 最终成品的质量，必须加以去除。目前对于银杏花粉 遗植物之一，有裸子植物活化石之称[1] ，银杏花粉是银 多糖中脱色的研究还鲜见报道，因此本实验主要应用物 杏的配子体，富含黄酮、多糖、内酯类化合物等[ 2 - 3 ] 。 理方法对银杏花粉多糖进行脱色，依据脱色的综合效果 多糖是由多个单糖分子经糖苷键结合而成，是构成生命