京尼平苷对体外培养大鼠关节软骨细胞活化作用的实验研究-外科学专业论文.docxVIP

三峡大学硕士学位论文 三 峡 大 学 硕 士 学 位 论 文 PAGE III PAGE III Abstract Objective To study the effect of Geniposide on the cell morphology、proliferation、 differentiation of rat chondrocytes in vitro.And to provide scientific basis of treating osteoarthritis with Geniposide. Methods 1.The chondroeytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultrastructure，with immuocytochemistry and RT-PCR for type II collagen, with the method of MTT assay for grow curve and proliferation,2.The 3rd passage chondrocytes were divided into blank group，different concentration Geniposide groups and were sequently passaged to 4th generation. Inunuoeytoehemistry for type II collagen，MTT assay for proliferation,flow cytometry for cell life cycle and proliferation index，RT-PCR and Western blot for type II Collagen. Results 1.The method of enzyme digestion is practicable for better activity cells， which were identified that had good phenotype and differentiation. 2. Geniposide has better activation function on several respect such as promoting chontrocyte proliferating （P0.05）, reducing cell content on G1 phase and increased S phase（P0.05）, increase the collagenⅡmRNA expression (P0.05)and collagen Ⅱsynthesi. Conclusion The method of enzyme digestion is practicable and can offer optimal chondrocytes.2 Geniposide has significant anti-senenscence function.3. Geniposide is one of effective components of gardenia in treating osteoarthritis. Key words: osteoarthritis Geniposide Chondrocyte II collagen PAGE IV PAGE IV 目 录 英文缩略词表………………………………………………………………1 引言…………………………………………………………………………2 材料与方法…………………………………………………………………3 结果…………………………………………………………………………8 第一部分: 大鼠软骨细胞体外分离、培养与鉴定 第二部分:京尼平苷对体外培养大鼠软骨细胞的活化作用 讨论…………………………………………………………………………14 结论…………………………………………………………………………19 参考文献……………………………………………………………………19 综述…………………………………………………………………………23 后记…………………………………………………………………………30 三峡大学硕士学位论文 三 峡 大 学 硕 士 学 位 论 文 PAGE 1