野油菜黄单胞菌6磷_酸葡萄糖脱氢酶基因的初步分析.pdfVIP

26 3 2007 9 Vol.26, No. 3 Journal of Guangxi Agric.and Biol.Science Sept . , 2007 : 1008- 3464 ( 2007) 03- 0190- 06 6-a 1,2,3 1,2 1,2 1,2 谭旖宁 , 马增凤 , 陆光涛 , 唐纪良 ( 1 , 530005; 2, 530005; 3, 530227) : , 8004 , 6-, XC1977 XC4082, 36. 3% , pK 18 mob 1977 4082 , , , 1977 XC XC XC XC4082 , , XC1977 56. 3% , XC4082 , 8004 z wf , XC1977 : ; 6-; : Q754 : A -6- Elementary analysis of glucose phosphate dehydrogenase genes of Xanthomonas camp estris 1, 2, 3 1, 2 1, 2 1, 2 T AN Yi-ning , MA eng-feng , LU Guang-tao , T ANG Ji-liang ( 1College of Life Science and T echnology, Guangxi U niversity, Nanning 530005, China; 2Guangxi Key Laborat ory of Subtropical Bioresource Conservation and Utilization Nanning, 530005, China; 3Guangxi Vocational Technology College Nanning, 530227, China) Abstract: X anthomonas camp estr is pv. c amp estr is ( X cc ) is used to produce xanthan gum in ferment industry. Examination of the complete genome sequence of X cc 8004 strain revealed that two z wf genes, one was named XC1977, the other was XC4082, which encoding glucose-6-phosphate dehydrogenase, were harbored. Sequence analysis showed that the similarity was only 36. 3% between the two genes. The two genes were inactived respectively by using suicided plasmid pK18mob and nopolar integrated mutants were attained Analysis showed that the extracellular polysaccharide yield. of the XC1977 mutant decreased 56. 3% compared to that