减味乌梅丸对于2型糖尿病患者血清氨基酸谱影响的实验研究中医内科学专业论文.docxVIP

减味 减味乌梅丸对于 2 型糖尿病患者血清氨基酸谱影响的实验研究 陕西 陕西中医学院 2013 届硕士学位论文 PAGE PAGE 13 PAGE PAGE 10 Less flavor WuMeiWan experimental studies for determining amino acids in the serum of patients with type DM Purpose: This issue through using high performance liquid chromatography (HPLC) analysis was used to detect diabetes mellitus (DM) patients and normal serum amino acid metabolism spectrum, observed diabetes and normal serum amino acid metabolic characteristics, and thus reduce odor of diabetic patients taking ebony pills before and after taking the metabolism of amino acids in serum distribution, evaluation less flavor ebony pill efficacy for people with diabetes, and explore the effects of type 2 diabetes prescription possible mechanism. Methods: This topic by using o-phthalaldehyde (OPA) pre-column derivatization high performance liquid chromatography (HPLC) was established seven kinds of amino acid metabolism in serum relative quantitative spectral analysis method. The experiment was divided into three groups, healthy group of 10 patients in the control group of 10 patients, less flavor ebony pill group 10 cases, a course of treatment for seven days, the three amino acids in serum metabolites measured spectra were analyzed. Three groups were conducted on fasting blood samples were taken before administration, less flavor ebony pill group, respectively, after a course of the meal 1h, 2 h, 3 h for taking blood samples. Three groups were detected in a blood glucose before and after treatment. Chromatographic conditions: fluorescence detector, mobile phase (A): acetonitrile; mobile phase (B): 20 mmol / L Na2HPO4, (2mol / L sodium hydroxide solution to adjust pH 7. 8); (gradient :0-9min, 15%; 9-25min, 15% -25%; 25-33min, 25% -35%; 33-45min, 35%), 45 min full within seven kinds of amino acids are well separated. Conclusion: Normal subjects, patients with type 2 diabetes and reduce odor ebony pill drug intervention patients, seven total concentration of amino acids in normal an