假单胞SJT25所产新颖化合物2-庚基-5-己基呋喃-3-羧酸的发现及其抗植物病原菌效果测定-生物化工专业论文.docxVIP

上海交通大学硕士学位论文摘要 上海交通大学硕士学位论文 摘要 万方数据 万方数据 假单胞 SJT25 所产新颖化合物 2-庚基-5-己基呋喃-3-羧酸的发现 及其抗植物病原菌效果测定 摘 要 近年来植物病原菌感染已经引起了大规模的农作物减产，并成为现代 农业的主要威胁之一。寻找新的天然抗生物质是植物保护领域克服病原菌 感染的重要而有效的途径。 本研究采用生物活性导向的筛选方法，从上海市闵行郊区水稻根际分 离到一株具有拮抗植物病原菌活性的假单胞菌株SJT25。因该假单胞菌株 能利用1-氨基环丙烷-1-羧酸为唯一氮源，故其属于植物根际促生细菌。对 该菌株进行摇瓶发酵，从其发酵液中分离到一种对植物病原菌有较强拮抗 活性的化合物。通过紫外、红外、质谱、核磁共振等图谱分析，鉴定其结 构为2-庚基-5-己基呋喃-3-羧酸（HHCA），通过SciFinder数据库比对，确 认该HHCA结构在国内外尚未报导，是一种新的化合物。针对该化合物的 抗植物病原菌效果测试表明，它对几种典型病原菌均显示出较强的拮抗活 性：对西瓜枯萎病，水稻纹枯病，西瓜炭疽病菌，番茄灰霉病和丁香假单 胞菌番茄致病变种的最小抑菌浓度（MIC）分别为2 μM、10 μM、20 μM、 25 μM和50 μM。并且也在水稻和甜瓜两种植物叶片上验证了HHCA的病原 菌拮抗能力。以上结果显示，2-庚基-5-己基呋喃-3-羧酸是一种很有潜力的 新颖候选农用药物。 关键字：根际促生细菌, 假单胞, 新颖化合物, 植物病原菌, 拮抗 III A NOVEL COMPOUND 2-HEPTYL-5-HEXYLFURAN-3-CARBOXYLIC ACID PRODUCED BY A NEWLY ISOLATED PSEUDOMONAS SP. SJT25 AND ITS ANTI-PHYTOPATHOGEN ACTIVITY DETECTION ABSTRACT In recent years, phytopathogen infection has caused a large number of crop failures and becomes one of the major threats to modern agriculture. Searching for new natural antimicrobial substances is a very important and potential way for protecting crops against phytopathogen infection. In the present study, Pseudomonas sp. SJT25 which strongly antagonized plant pathogens was isolated from rice rhizosphere soil using a bioactivity-guided approach. A novel compound was isolated from the fermentation broth of Pseudomonas sp. SJT25, and it was identified as 2-heptyl-5-hexylfuran-3-carboxylic acid by analysis of UV, IR, MS and NMR. The novel compound showed strong phytopathogen antagonism. Its MICs for Fusarium oxysporum, Rhizoctonia solani, Colletotrichum orbiculare, Botrytis cinerea and P. syringae pv. tomato were 2, 10, 20, 25 and 50 μM, respectively. The HHCA was also demonstrated to show effective inhibition to fungal infection in rice and melon leaves. These results suggested that HHCA might be a new promising candidate of antibiotic agent. KEY WOEDS: PGPR, Pseudomonas sp., novel compound, phytopathogen, antagonism IV 上海交通大学硕士学位论文目录 上海交通大学硕士学位论文 目录 目 录 学位论文原创性声明………