镍诱导对小麦幼苗SOD活性和同工酶的影响-河南农业科学.PDF

DOI :10.15933/j.cnki .1004 -3268.2009.10.012 河南农业科学 SOD ＊ 王素平, 王　勤, 王启明 ( , 476000) :以小麦幼苗为试材, 采用营养液水培, 研究了不同浓度(5、10、20、50、100、130 mg/L )镍处理 对小麦幼苗植株生长、SOD 活 和SOD 同工酶谱的影响。结果表明, 镍处理下小麦幼苗SOD1、 SOD2 和根系SOD1 酶带增强, 其他SOD 同工酶酶带减弱, 叶片和根系SOD 活 均下降, 小麦幼 苗生物积累量与SOD 活 存在较强的正相关, 提示镍处理下, SOD 酶带减弱及其引起的SOD 活 下降是导致小麦幼苗生长受抑的 一个重要原因。 :镍;小麦;浓度;SOD;同工酶 :S512.1 　　:A 　　:1004-3268(2009)10-0053-05 Nickel-induced Changes in SOD A cti ity and Isoforms of Wheat Seedlings ＊ WANG Su-ping, WANG Qin, WANG Qi-ming (Department of Life Science, Shangqiu Normal College, Shangqiu 476000, China) Abstract:Taking the seedlings of w heat (Tr it icum aestivum L.)as experimental material, the effects of Ni2+ at different concentration (5, 10, 20, 50, 100, 130mg/L)on grow th, SOD acti ity and isoform s w ere studied by the method of hydroponics.The results show ed that under nickel treat- ment, the SOD1 and SOD2 isoforms in leaf and SOD1 isoform in root strengthened ob iously com - pared w ith the control, w hile the other SOD isoforms w eakened ob iously .SOD acti ities in leaf and root of nickel-treated w heat seedlings decreased .And a positi e correlation w as found closely between biomass and SOD acti ity .This indicated that the grow th retardation of w heat seedlings exposed to ex cess nickel w as partly attributed to the low er SOD acti ities w hich w as caused by the w eakened SOD isoform s. Key words:Nickel;Wheat ;Concentration;SOD;Isoenzyme 　　, 、 Ni 。 、, 。 4 2 [4 ～8] 2×10 hm ,