甲状旁腺素对心脏和冠脉循环的作用-生理学-心脏电生理学专业论文.docxVIP

中文摘要甲状旁腺素对心脏和冠脉循环的作用 中文摘要 甲状旁腺素对心脏和冠脉循环的作用 甲状旁腺素parathyroid hormone(PTH)是由甲状旁腺主细胞分 泌的肽类激素，现认为甲状旁腺素除通过对肾、骨的效应来维持血液 中钙的水平以外，还可影响多种细胞功能。 本课题着重研究甲状旁腺素对心血管功能的影响，阐明甲状旁 腺素的另一重要生理功能，并初步探讨其作用机制。运用膜片箝、细 胞内微电极记录技术及Langendorff灌注方法，从离子通道、细胞动 作电位和离体心脏三个水平来观察甲状旁腺素的心血管效应。实验结 果显示：1)PTH可使兔心窦房结细胞自律性增强，心率加快，动作 电位4期自动除极速率增加，阈电位上移。2)钙通道阻断剂nifedipine 可减弱上述效应。3)膜片筘实验显示，PTH可使起搏离子流If向正 电位方向移动，增强激活过程。4)PTH可使离体心脏收缩力呈剂量 依赖性增强，20nmol／L PTH可使心肌收缩力增加近50％。5)PTH 使离体心脏冠脉流量增加，呈剂量依赖式。20nmol／L PTH可使冠脉 流量增加近56％。结果表明PTH具有增加心率、心肌收缩力和冠脉 流量的作用。 尽管循环血液中的PTH水平是相当低的(～IOPM)。但是，在 一些疾病状态下，血液中的PTH水平可升高，例如原发性甲状旁腺 功能亢进、慢性肾衰叭2删等，常会引起各种有害的心血管效应n- 。 因此，对PTH的心血管效应的研究为临床在诊治这些疾病过程中， 加强对心血篱功能的保护提供实验依据。关键词：甲状旁腺索；窦腾结；动作电位；膜片筘；越搏离子流：冠 加强对心血篱功能的保护提供实验依据。 关键词：甲状旁腺索；窦腾结；动作电位；膜片筘；越搏离子流：冠 脉流量 ABSTRACTThe ABSTRACT The effects of parathyroid hormone on heart and coronary circulation Parathyroid hormone(PTH)，a peptide，is secreted from the parathyroid gland．The main physiological significance of the hormone is to maintain homeostasis of Ca ions in the body．Though the target organs of the PTH are bone marrow and kidneys，it has been reported that PTH have some other effects on cardiomyocytes This subject focuses on the effects of PTH on automaticity of Sinus Node，contractivity of cardiac muscles and coronary flowing Conventional microelectrode，perforated patch and Langendorff perfusion techniques were used to study its effects on ion channel，cell and organ levels．The results are as follows：1)PTH could increase the heart rate by 1 7％because of its fastening of the spontaneous depolarization rate in phase 4．21 Nifedipine，a calcium channel blocker，could partially reverse the effect produced by PTH．3)The activation of pacemaker current If is increased by moving the I-V curve towards positive direction under the influence of PTH．41 PTH exerts a positive inotropic effect On isolated rabbit heart with a dose．dependent manner and PTH 20 nmol／L could increase the contraction force by 50％．5)When the heart beating was under control at a rate of 200