酵母基因组中钙离子平衡相关基因的初步研究-微生物与生化药学专业论文.docxVIP

ABSTRACT In eukaryotic cells，the calcium ion (Ca如) functions as a ubiquitous intracellular messenger regulating a myriad of biological processes such as cell proliferation ， muscle contraction ，programmed cell death. Calcium homeostasis is highly regul剖ed +to maintain Ca2 + levels within optimal concentration ranges in the cytosol and other organelles. In yeast，there are also many unknown mechanisms ωbe unidentified in +、 the Ca2 +  +homeostasis sys阳m，so we screen the genes related to Ca2 +  signaling pathway via a high-throughput experiment. A detailed knowledge of calcium homeostasis systems will likely contribute to new，more effective drug therapies for 1 the tre创ment of fungal disease，as well as autoimmune and neurodegenerative disorders. From a genome-scale genetic screen，we have identified 37 calciumsensitive gene mutations in 81αccharomyces cerevisiae. We find that twelve mutations are strongly sensitive ωcalcium and ten mutations are moderately sensitive to calcium. Functional analysis indicates 由创 26% of these identified genes are involved in the vacuolar protein sorting (VPS) pathway ，14% in the PHO pathwáy ，14% in responseωhigh +osmolar stress，and 11% in the regulation of V-ATPase function. In addition ，30% of gene products locate in the cytoplasm ，15% in the vacuole，12% in the nucleus ，and 9% in ER. We have also examined the whole cell Ca2 contents of the 37 + +strains and found that the whole-cell Ca2 + contents of 9 strains are 1.5 times higher than that of the wild type BY4743. 4民 KEY WORDS: calcium， 8accharomyces cerevis邸 genon四-wide screening， +whole cell Ca2 + 、  contents 目录 第…章文献综述…………………………………………… J 1.1 酵母和酿酒酵母…………………………………… 1 1.1.1 酿酒酵母基因组结构…………………… 1 1.1.2 酿酒酵母作为模式生物… 2 1.1.3酿酒酵母基因缺失株文库……………………………………… .3 、1.2 钙离子平衡调控…4 、 1.2.1 钙离子平衡调控机理……………… 4 1.2.2 钙调磷酸酶酶………………… 5 1 1.2.3 RCANs: 钙调磷酸酣酶调控因子……… 7 1.2.4钙调磷酸酣酶的调控因子 HSPs…………………………………川9 1.2.5 钙调磷酸酶酶调控与真菌发病机制