秸秆固态发酵酒精的研究-食品科学专业论文.docxVIP

坝【学位论义 坝【学位论义 摘莹 摘 要 本文上要对秸秆I矧态发酵生产酒精所州的菌种和】‘艺路线进行_r初步的坩f究，得剑r以 F究成果： 秸千十经过揉搓粉碎等处理后，选取酸、碱和蒸汽爆破等二种方法进行项处理，综合考虑 纤维转化率、对设备的要求、环境污染等因素，确定顶处理采用蒸汽爆破法，住l 2MPa r持 J{、5min。 ， f利J_}j康氏木霉尉态发酵生产纤维素酶曲，酶曲的培养条件是：秸科粉：盍夫皮=1：1。I捌液比 =l：3，添加2％的硫酸铵，接种5％，30C培养84小时左右。＼】 ，＼ 利用电场诱导原生质体融合技术将产酒率高的菌株Sbl和耐高温但产酒率低的No．30进 行融合，培育耐高温酵母，以达到酵母发酵温度与纤维素酶解温度相一致。f通过对两亲株进 }j遗传标记，用3．5％蜗牛酶处理原生质体，在脉冲强度、宽度、次数为11KV、1011 S、2次 时进行融合，得到了可在45℃产酒精能力为7．28％的酵母融合株2。0 { 住同步固态发酵中，采用单因素及正交实验对发酵条件和工艺参数进行了研究。f在纤维 素酶曲用量421U／g(F队)，加水比1：2的情况下，接种酵母5％，45C发酵24小时可产生酒 精7 2％(按秸秆干基计算)。J ／、 关键词：秸秆i固态发酵i酒精i耐高温酵母i纤维素酶曲j AbstractThe Abstract The procedure for obtaining ethanol from corn straw by Simultaneous Solid—Fermentation was studied in this paper． The influence of the acid hydrolysis＆enzymatic hydrolysispress boiling and explosion showed the press boiling and explosion fit straw pretreatment It was in l 2 MPa and 5 minutes The factors including medium，water contents，nitrogen sources，cultural time and cultural temperature were described for producing cellulase by the mutant strain of Trichoderma koningii then The results showed that the optimum conditions of solid fermentation are straw：wheat bran =】：j，the rate 1：3 of solid and liquid，(NH4)2S042％，30℃cultivation 84 hours． Protoplast fusion between S cerevisiae No．30 and S．bayanus 1 had been carried for resolving the difference of enzyme and yeast in Simultaneous Sacchrification and Fermentation．Sbl is a high alcohol yield yeast but can’t grow at 45℃．However No．30 can grow at 45C but has a lower alcohol yield The best intensity,width，inter and frequency of the electrofusion impulse were 1 1 Kv，1 0 p S，2 respectively．The results showed that the fosant greatly expressed the properties of parent strains in thermotolerance and ethanol yield The fusant 2 has a high ethanol yield(7．28％)using glucose as substrate at 45℃ The optimum conditions of Simultaneous solid—state fermentation were investigated by using orthogonal test．We got 7 2％ethanol from corn straw(dry)in the condition of 421U／g