胶陀螺多糖的提取纯化及结构研究-生物化学与分子生物学专业论文.docxVIP

中文摘要从胶陀螺(Bulgaria 中文摘要 从胶陀螺(Bulgaria inquinans)中分别以75％乙醇回流、冷水、 热水浸提得到三个粗糖级分BP I、BPlI、BPIII，BPllI经乙醇分级后得 到三个级分BPI]la、BPlUb、BPIIIc，其中BPIIIb冻融除杂后经Sepharose cL一4B柱层析，DEAE—Sephadex A一25柱层析和醋酸纤维素薄膜电泳检 验，BPIIIb为单一狭窄对称峰且电泳呈单一谱带。纯度鉴定表明BPIIIb 在分子量大小和极性上都较为均一。 组分分析表明BPIIlb的单糖组成为Glc、Man、Gal，摩尔比为 3．73：1．OO：0．76。 BPIIlb的结构分析采用了部分酸水解，碘反应，高碘酸氧化，SmJth 降解，G．C．分析，甲基化分析等方法，甲基化产物经永解、还原、乙 酰化后用G．C．-M．S．联机分析。 (分析结果表明：BPIIIb多糖结构分枝少，平均每8个单糖残基中 有l、个分枝。(1—6)Glc，(1—6)Man构成主链的核心部分，在0—3 处有分枝。支链和主链的边缘由较多的不可氧化(1—3)G1c，(1—3) Man和较少的可氧化(1—6)Gal和(1—6)61c，(1—6)Man构成。 分枝点糖残基有(1—3，6)Glc和(1—3，6)Man，其中大多数为(1—3， 6)Glc。上 关键词l胶陀螺／多糖分气纯恢结构研瞅 AbstractThree Abstract Three kinds of crude polysaccharides BP I，BP II，BPm were extracted from Bulgaria inquinans by 75％ethanol，cool water,boiling water respectively．After ethanol grading，the crude polysaccharide BP 11 was divided into three components BPIII文BPIIIb and BPIIIc．Then the impurity in BPIIIb were eliminated by freezing and thawing repeatedly． Tested by SepharoseCL一4B Chromatogram，DEAE-Sephadex A-25 Chromatogram and elec仃ophoresis，BP 1II b were proved to be homogeneous polysaccharide on molecular weight and polari够as well． GC．analysis showed that BPⅢb were made up of Olc．Man and Gal． which mol ratio were 3．73：1．00：0．76． Partial hydrolysis with acid，Periodate oxidation,Smith degradation, I．R．，and Methylation were used oil the structure analysis of BPHIb．After acetylation，themethylatedproductionweleanalyzedbyG．C．．M．S． The results revealed that BPIIIb were a kind of less branched structure． On average，there is I branch in each 8 main chain residues．11main chain is composed of(卜-6)4inked-Glc and(1—6)-linked-Man，which is substituted at 0-3．The edge of the main chlii】n and the side chain were mainlymadeupof(1—3)Gleand(1—3)Man． Key words：gu]garia inquinans Polysaccharide Separation Purification Structure Study 独创性声明本人声明所呈交的学位论文是本人在导师指导下进行的研究工 独创性声明 本人声明所呈交的学位论文是本人在导师指导下进行的研究工 作及取得的研究成果。据我所知，除了文中特别加以标注和致谢的地 方外，论文中不包含其他人已经发表或撰写过的研究成果，也不包含 为获得东北师范大学或其他教育机构的学位或证书而使用过的材