金雀异黄素对体外小鼠胚胎肢芽软骨生长发育的影响-营养与食品卫生学专业论文.docxVIP

中文摘要金雀异黄素对体#t-d,鼠胚胎肢芽 中文摘要 金雀异黄素对体#t-d,鼠胚胎肢芽 软骨生长发育的影响 目的：利用小鼠胚胎肢芽体外培养方法，研究金雀异黄素对软骨发育分化的 影响。 方法：取出孕12天小鼠胚胎肢芽，露旋转装置上体外培养3天或6天，同 时加入10 9mol／L～104mol／L会雀异黄素，并设对照组，收获后的肢芽用图 象分析仪测量软骨面积，同时，用原位杂交的方法测量肢芽软骨细胞II型胶 原mRNA及BMP．2 mRNA的表达水平。结果用SPSS 10．0 for Windows进 行单因素的方差分析。 —飞／结果：1．前肢培养3天与6天后，10．6mol／L与10‘5mol／L组的爪骨面积、总 骨面积显著高于对照组。后肢培养3天后，长骨面积、总骨面积／总面积在 10。5mol几组显著高于对照组：6天后，在101mol／L与10“mol／L组总骨而积 ／总面积显著高于对照组。 2．体外培养3天的肢芽在10‘6mol／L与104mol／L组的II型胶原mRNA及 BMP一2 mRNA阳性表达密度与积分光密度显著高于对照组；体外培养6天 的肢芽在10’6mol／L与10巧mol／L组的II型胶原mRNA及BMP．2 mRNA阳性 ＼／ 表达丽积与积分光密度显著高于对照组。巧 ／ 结论：金雀异黄素在一定浓度下能刺激胚胎肢芽软骨细胞BMP．2 mRNA及 II型胶原mRNA的表达，促进发育中软骨面积的增加，具有刺激胚胎软骨 发育分化的作用。 关键词：余雀异黄素胚胎肢芽软骨面积II型胶原BMP．2 AbstractEffect Abstract Effect of Genistein on the Cartilage Development of Mouse Limb Bud{n vitro Objective To study the effect of gen J stein on the carti】age devel opment of mouse Iimb bud in vitro，the method of the CUlture of fetal limb bud in vitro iS used． Methods The fetal limb bud of 12一day gestational period were cul tured in the rotating apparatus in vitro for 3 or 6 days．Geni stein from 10一mol／l， to 10。smol／L were added to the medium．The area of the cartilage were measured through image analyzer．The expression of type 2 c01]．agen mRNA and BMP-2 mRNA were determined by in situ hybridization．SPSS 10．0 for Windows were used to process the one—way ANOVA． Resul ts 1．For the forel imb，in the i01 mol／L and 10一mol／L group，the area of the paw and the bone were significantly 1arger than that of the contr01．As for the hindlimb，after 3 days，the area of 10ng bone and the ratio of the bone to the whole 1imb bud in the 10一mol／L group were Significantly larger than that of the contr01．After 6 days，in the 10—7mol／L and 10—5mol／L group，the ratio of the bone to the whole limb bud were significantly larger than that of the contr01． 2．After 3 days，the density and IOD of posi rive expression of type 2 col lagen mRNA and BMP一2 mRNA of chondrocyte in the lO“mol／L and lO 6mol／L group were signi ficant