金匮肾气丸及其与微营养素并用对致衰小鼠雄激素水平的影响及抗氧化作用的研究-营养与食品卫生学专业论文.docxVIP

浙江大学硕十学位论文Study 浙江大学硕十学位论文 Study the effect of the Jinkuishenqiwan and miCronUtrlentS 0n andr02en leVel and antl一0Xldatlon ln male mice induced by D·galactose SuperVisor： Baoshuj ing Postgraduate： Fenglei Abstract objectiVe：Tb obsen，e the ef．fects of Jinkuishenqiwan and micronutrients on androgen leVel and anti—oxidation in male mice induced by D-galactose． Methods： 1．Grouping： Sixty ICR male mice were randomly divided into two groups according to their weights：1 2 in the nomal comrol group and 60 in the senile model group． 2．Establishing the senile model： The mice in the senile model group were giVen D-galactose through subcutaneous i匈ection and the ones in the control group injected equal 0．9％sodium chloride once per day．All the mice had common feedstuff and draI墩water freely．ARer 6、veeks，confimling if the model is successfully made by checking the actiVity of GHS·PX and SOD in the blood． 3． Experiments： The successfully made senile model group was further diVided into five subgroups：model control group；jillkuishenqiwan in low dose group； ．3- 浙江大学硕上学位论文j 浙江大学硕上学位论文 j inkuishenqiwan in high dose group；j iIll【uishenqiwan in low dose plus micronutrient group；jinkuishenqiwall in high dose plus micronutrient group． Each group was treated with distilled water，80mg／ml j iIll(uishenqiw髓liquid， 1 25mg／ml jinkuishenqiwan liquid， 80mg／ml jinkuishenqiwan liquid plus micronutrients， 1 25mg／ml jinkuishenqiwan liquid plus micronutrients respectiVely．The mice in fiVe groups had common feedstuff and drank water freely．After 6 weeks，checking the indices as follows，the semm androgen leVel，the actiVity of GSH-PX and SOD in blood，lung，liver，brain and heart． Result： 1·The ef-fbct of the jinkuishenqiwan and micronutrients to the androgen、 SOD and GSH-PX leVel in blood of the senile male mice induced by D—galactose：The androgen、 SOD and GSH—PX levels of the senile mice in the four experimental groups are aU increased in difIferent degree．The indices in low dose are higher group than thos