结直肠癌中SOX9的表达及临床意义-内科学专业论文.docxVIP

Expression of SOX9 in colorectal cancer and clinical significance Abstract Objective To determine the expressions of SOX9 in colorectal cancer (CRC), and analyse the relationship of SOX9 expressions with clinico-pathological parameters, and possible mechanism. Methods The tissue samples were obtained from 189 CRC patients who underwent curative surgery at the First People’s Hospital of Yancheng from May. 2013 to Oct. 2013. Clinical data were collected. In all patients, the CRC were confirmed histologically, based mainly on the examination of sections stained with hematoxylin and eosin. Expression of SOX9 was detected by streptavidin peroxidase immunohistochemistry techniques. The intensity was scored as 0 (no immunostaining), 1 (weak immunostaining), 2 (moderate immunostaining), or 3 (strong immunostaining). The percentage of nuclear positive tumor cells was scored as 0 (none), 1 (10%), 2 (10 to 50%), 3 (51 to 80%), or 4 (＞80%). Multiplication of the scores for intensity and percentage resulted in a staining index ranging from 0 to 12. A staining index of 0 to 6 was defined as low and a staining index of 8 to 12 as high expression. Results The immunohistochemistry examination showed that the positive rates of SOX9 protein was 37.57% (71/189) in 189 CRC tissues, SOX9 expression was positively correlated with the TNM stage, tumor size, invasion degree and lymph node metastasis(P0.05), but not associated with gender, age, location, pathologic classification and differentiation(P0.05). Conclusion SOX9 might play an important role in tumorigenesis of CRC. In CRC tissues, SOX9 expression might have an important role in the aggressiveness and lymph node metastasis. Written by: Zheng-Jiang Wang Supervised by: Chun-Fang Xu 目 录 前 言.....................................................................................................................................1 材料和方法.......................................................................................................