金纳米放大压电重金属离子检测研究分析化学专业论文.docxVIP

重庆大 重庆大学硕士学位论文 英文摘要 PAGE PAGE VI ABSTRACT Undecomposebel, accumulateing in the organisms for a long time and having a high toxicity in a trace content, heavy metal is a harmful pollutant. Once it accumulate in human body through the food chain, it cause various diseases,even affect the next generation. Therefor, heavy metal detection is very important for medicine, food and environment. Traditional trance heavy metal detection methods which is based on expensive instrument, wasting of money and time , unsensitive or undetectable for some metal ions ,and need for specialist operator are not competent for these days. Thus new methods with simple instrument ， speediness and high sensitivity is need for qualitatively and quantitatively detect heavy metals in samples. Herein, A new and novel detection method for heavy metal ions has been developed using quartz crystal microbalance(QCM) as a sensor and gold nanoparticles (GNPs) as a signal amplifier. Resonant frequency shift of QCM caused by the selfassembleing of heavy metal ions and nanoparticles on the electrode was detected for heavy metal determination.Metal ions were captured onto the binding reagent modified QCM surface, the same material modified gold nanoparticles are then introduced to bind the surface-captured metal ions. The metal ions, binding reagent modified gold substrate and GNPs formed sandwich nanostructures by binding effect between metal ions and binding reagent, and cause a remarkable QCM resonant frequency decrescence as a detection signal. The present thesis is composed of the following three parts: ① Gold nanoparticles were synthesized by reduction of HAuCl4 with trisodium citrate. TEM simage show that synthesized gold nanoparticles are spherical dispersive and equirotal with an average diameter of 10±0.8nm .The ultraviolet-visible spectrum of gold nanoparticles show a sharp and narrow absorption peak appears at 517.5nm.The stability of uncapped GNPs,MBA capped GNPs and oligonucleotide