假松口蘑的SCAR特异性分子标记-江苏农业学报.PDF

江苏农业学报(JiangsuJ.of Agr.Sci. ),2015,31(4):906~909 906 http:/ / 兰摇 西,吴松权,金美玉,等. 假松口蘑的SCAR特异性分子标记[J].江苏农业学报,2015,31(4):906鄄909. doi:10.3969/ j.issn.1000鄄4440.2015.04.031 假松口蘑的SCAR特异性分子标记 兰摇 西,摇 吴松权,摇 金美玉,摇 全雪丽 (延边大学农学院,吉林 延吉 133000) 摇 摇 摘要:摇 由于假松口蘑与松口蘑外观与口感相似,常被混入松口蘑中销售,严重影响松口蘑品质。 本研究基于 SRAP分子标记从300对引物组合中筛选出1对假松口蘑具有特异性的引物,并将这对引物的扩增产物进行克隆、 测序以及重新设计引物,成功构建了假松口蘑的SCAR特异性分子标记(502 bp)。 通过进一步验证,该SCAR标记 只在假松口蘑个体中出现,表明所建立的SCAR标记可在分子水平上快速、准确地鉴定出假松口蘑。 关键词:摇 松口蘑; 假松口蘑; SRAP; SCAR 中图分类号:摇 Q785摇 摇 摇 文献标识码:摇 A摇 摇 摇 文章编号:摇 1000鄄4440(2015)04鄄0906鄄04 Development of SCAR (sequence characterizedamplifiedregions) specific markers of Tricholoma bakamatsutake LAN Xi,摇 WU Song鄄quan,摇 JIN Mei鄄yu,摇 QUAN Xue鄄li (College of Agriculture,Yanbian University,Yanji 133000,China) 摇 摇 Abstract:摇 Quality of Tricholoma matsutake has been seriously influenced because it is often mixed with T. baka鄄 matsutake due to their similar appearance and taste. In this study,a pair of primers specific for T. bakamatsutake was screened from 300 pairs based on sequence鄄related amplified polymorphism (SRAP) molecular markers. Then a SCAR marker (502 bp)for T. bakamatsutakewassuccessfully developedby cloning,sequencingandprimersredesigning. The SCARmarkerwasonly presented inthe individualsof T. bakamatsutake. In conclusion,the SCARmarker could quickly and accurately identify T. bakamatsutake at molecular level. Key words:摇 Tricholoma matsutake; T. bakamatsutake; sequence鄄related amplified polymorphism (SRAP); sequence characterized amplified region (SCAR) [4] 摇 摇 松口蘑 (Tricholoma matsutake)被誉为菌中之 治疗糖尿病,并具有抗癌作用 。 假松 口蘑 [1鄄2] [3] 王 ,目前还不能实现人工栽培 。