课件：肾干-祖细胞枯竭与肾纤维化.ppt

* Figure 4. CD133CD24PDX cells, but not CD133CD24 PDX or CD133-CD24 cells, reduce proteinuria and improve glomerular and tubulointerstitial injury in SCID mice affected byadriamycin-induced nephropathy. Albumin/creatinine ratio as measured at day 7 in adriamycin-treated mice that received saline(n 12), CD133CD24 (n 6), CD133CD24PDX (n 6),and CD133CD24PDX (n 12) bulk of cells or two representative clones of CD133CD24PDX cells (n 3 for each clone). Data are expressed as mean SEM. a versus b, a versus c, b versus c, d versus e, d versus f, e versus f, NS; a versus d, b versus d, c versus d, P 0.001; a versus e, a versus f, P 0.01; b versus e, b versus f, c versus e, c versus f, P 0.05. (B) Time course experiments of albumin/creatinine ratio as measured in healthy (black square) or adriamycin-treated mice that received saline (red circle) or CD133CD24PDX cells (blue triangle).Red arrow points to the day of adriamycin injection. Black arrows point to the days of CD133CD24PDX cell injection. Data are expressed as mean SEM. (n 12 mice at each time point for each group of treatment). P 0.0001 between the two groups of treatment (ANOVA for multiple comparisons). (C) Left: PAS staining of renal cortical sections of mice with adriamycin-induced nephropathy treated with CD133CD24PDX cells; objective 20. Right: High-power magnification of a glomerulus; objective40. (D) Left: PAS staining of renal cortical sections of mice with adriamycin-induced nephropathy treated with saline.; objective 20. Right: High-power magnification of a glomerulus; objective 40. (E) Quantitation of glomerular and tubulointerstitial injuries in renal cortical sections of saline-treated and CD133CD24 PDX treated mice after the induction of adriamycin nephropathy. Data are expressed as mean SEM. g versus i and h versus j, P 0.001. * Hypothetical diagram for kidney regeneration by different types of renal and extrarenal progenitors. CD24+CD133+ renal progenitors (red) are localized at the