优质细毛羊羊毛细度的候选基因分析-遗传.PDFVIP

HEREDITAS (Beijing) 2007 1 , 29(1): 70―74 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0070 1 2 3 4 2 刘桂芬 , 田可川, 张恩平, 黄锡霞 , 张艳花 1. , 100094; 2. , 830000; 3. , 712100; 4. , 830052 : 采用 PCR-SSCP 的分子标记技术, 选择编码羊毛纤维组成蛋白基因中的KAP1.1 、KAP1.3 的部分序列、 KAP6.1 的外显子区作为候选基因, 通过对其多态性的研究, 探索将该基因作为候选基因来间接选择羊毛细度 性状的可行性。得出其中在角蛋白辅助蛋白的多基因家族中的高硫蛋白辅助蛋白基因(KAP1.1 、KAP1.3) 中, 位 点W08667 与羊毛细度有显著的相关性(P0.05) 。在甘氨酸酪氨酸角蛋白辅助蛋白中, 外显子位点 W06933 的 AA 基因型和BB 基因型与羊毛细度之间有显著的相关性(P0.05) 。 : 优质细毛羊; 羊毛细度; 候选基因; PCR-SSCP Candidate gene analysis of high quality merino sheep 1 2 3 4 2 LIU Gui-Fen , TIAN Ke-Chuan , ZHANG En-Ping , HUANG Xi-Xia , ZHANG Yan-Hua 1. College of Animal Science and Technology, Chinese Agricultural University, Beijing 100094, China; 2. Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830000, China; 3. College of Animal Science and Technology, Northwest Sc-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China; 4. College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China Abstract: Partial sequences of wool fiber constituent genes KAP1.1 and KAP1.3 and the exonic sequence of the KAP6.1 gene were chosen for polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis to assess their ability as candidate genes during indirect selection for fine wool traits. Results show that locus W08667 in the genes (KAP1.1, KAP1.3) which code the high sulfur protein associated-protein of keratin associated-protein family is significantly correlated with