广东家庭聚集性登革3型病例特征及其病毒株全基因组序列研究-内科学 传染病专业论文.docxVIP

广州医学院硕士学位论文广东家 广州医学院硕士学位论文 广东家庭聚集性登革 3 型病例特征及其病毒株全基因组序列分析 PAGE PAGE 4 The characteristics of family-clustered dengue fever cases and analysis of full genomic sequence of the dengue virus type-3 strain in Guangdong Major: Infectious Disease Master Candidate: Weize Zhou Supervisor: Professor Fuchun Zhang Abstract Dengue fever （DF）is an acute infectious disease caused by dengue virus(DENV) infection, which is transmitted through the bite of infected mosquito .There are four serotypes of dengue virus（DENV-1、DENV-2、 DENV-3、DENV-4）.DF is now endemic in more than 100 countries in tropical and subtropical areas, and threatens the health of approximately 40% of the world’s population. The World Health Organization estimates that there may be around 100 million cases of dengue virus infection worldwide every year, resulting in approximately 500,000 cases of DHF/DSS and 25,000 deaths each year . Dengue mainly prevalent along the coast areas in China, while Guangdong province is the most affected area.Since 1978, there are dengue cases reported almost every year,all four serotypes of dengue virus have epidemics in Guangdong .But the research of DF in Guangdong was major in DENV-1 and DENV-2, however, little is known about DENV-3 and DENV-4. In the last ten years , the dengue endemic in Guangdong was mostly caused by DENV-1 ,no other serotype of DENV infected case was found . So we must pay close attention to clinical DF case, get the early specimen on time and isolate the wild strain of dengue virus, research and analyze the character of its clinic and pathology. All these are significant to understand the epidemic situation and its character, source of pathogen and control and prevention of DF in Guangdong Aim of this research: Analyze the clinical and laboratory characteristics of 3 cases of a family-clustered dengue fever in city of Guangzhou in Guangdong province August 2009 . Isolate dengue virus strain in serum from the patients, then sequence and analyze th