海产品及海水中有机氯农药及多氯联苯的前处理方法及应用研究-食品科学专业论文.docxVIP

宁波大 宁波大学硕士论文 海产品 海产品及海水中有机氯农药及多氯联苯的前处理方法及应用研究 万方数据 万方数据 万方数据 万方数据 The study on the application of pretreatment method for organochlorine pesticides and polychlorinated biphenyls in seafood and seawater Abstract Organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs), with characters of fat soluble, persistence, and hydrophobicity, etc, are major persistent organic pollutants, and they are hard to be degraded in the environment. Especially, some kinds of them exhibit properties of biological hormone and accumulation, and very small amounts of residual can bring compound disturbance to endocrine system of the organism and generate carcinogenic, teratogenic and mutagenic effects. Due to the existence of them in the offshore waters, they are easily enriched in marine products like fish, shrimp and shellfish, which resulted in a great harm to people’s health. Therefore, the trace detection of the OCPs and PCBs has been widely studied in marine science and food science in recent years. In the present study, we aimed at establishing multi-residue detection method for 17 kinds of OCPs and PCBs based on highly efficient sample pretreatment method, such as membrane extraction (MASE) and QuECHERs, combined with GC-ECD or GC-MS/MS. The establishment of the detection method for 17 kinds of OCPs and PCBs in seawater: membrane extraction technology is a kind of environmentally friendly sample preparation method with advantages of short time, less organic solvent being used, easily to realize automation and so on. The optimized parameters mainly included extraction reagent, time, RPM, temperature and the NaCl concentration. The results indicated that the best extraction reagent is n-hexane: acetone = 9:1 (v/v), the best extraction time is 60 min, the best extraction speed is 600 rpm , the best extraction temperature is 40°C, and the extraction effect of saturated NaCl is clearly superior to that of ordinary water. After detection by GC/ECD or GC/MS/MS detection, the r