固氮施氏假单胞菌RpoN和RpoS对鞭毛合成和趋化调控机制的分析-细胞生物学专业论文.docxVIP

PAGE PAGE VI Abstract In order to adapt to the complex environment, prokaryotes need to regulate the transcription and expression of specific genes by different sigma factors. In the evolution of microbial species，Sigma factors have developed diffirent structure and function. According to the differences of structure and function, Sigma factors can be divided into two families: σN and σ70. At present，σ70, σ54 and σ38are researched more deeply. Most studies are based on the level of bacterial transcriptome. Researchers discovered that the regulation of transcription factors is a fairly complicated process and is the coaction of many environmental factors. In prokaryotes, the multiple regulatory networks are used to regulate the response to external stimuli. Much evidence indicates that the transcription activation depends on the interaction of a multitude of transcription factors. In E.coli, we found a synergistic or antagonistic effect on the expression of certain specific genes. Whether there is the similar regulatory mechanism in E.coli and Pseudomonas is still unclear. T he rpoN del etio n m ut ant was constructed by gene replacement. There is the rpoS del etio n mutant in lab. Both of the two mutants were used to study the regulation mechanism of rpoN gen e an d rpoS gene on the flagella synthesis and the motility. The main results are as follows: The rpoN deletion mutant and the function complementary strain were constructed. In LB liquid medium, therpoNmutants of Pseudomonas stutzeri A1501grew very slowly. The cell density was only 50% of the wild type in stable phase. Gr owth o f Comp- rpoNstrain could recover to 80% of the wild-type. The results showed that the deletion of rpoN affected the growth of strain under normal conditions. On the contrary, the deletion of rpoS gene had no significant influence on the growth of A1501 in rich nutrition. Under the shock of 2M NaCl or 20mM H2O2, rpoS mutant were more sensitive than WT to the high osmotic pressure and