海南栽培荔枝与半野生荔枝种质的issr分析-果树学专业论文.docxVIP

ISSR ISSR analysis on CultiVated够pes and Semi—wildaccessions Lychee in Hainall ProVince Abstract There is only a naky Semi一、Ⅳildaccessions Lychee of China in YhngshaIl area (HainaIl province)，it is also me la昭est Semi一谢ldaccessions Lychee in world at 雠sent．低se pIecious Serni一讯ld Lychee germpbms arc being destroyed year by ycar，so it is urgent to save these precious n栅e gene．But，it is Very dimcult to protect all舢pl姗1s， 0n也e otller hand， it is easier to protect a s删l qu姐ti吼of nuclear ger瑚plasm． Consequently，genetic diVers毋of 80 accessions of Cultivated types Lychec锄d 115 accessions of Semi．丽ld Lychee we托stlldied by using ofISSR marker，in ordef to 口rovide academic baSes for protecting血e nuclear gerⅡlplasm of Semi—wild bchee accessions inY缸gshan． 皿e m两Drfesults are as f01lows： 1．The dens畸of S锄i-wild Lychee accessions inYangshan mat is 20．82 popl】1ation perlla．at mepresenttimeismoremuchIcssthanonethatis 8l populationper ha· before．Therefore，it is llrgem to save these precioIIs Lychee gemlplasm· 2．23 randoms ofISSR primers out of74、Vere ide呲ified haVe poly咖rphismbatlds．Total of 1 17 allelcs haVe obtained u，hen use mese polymorpllic markers to detect all materials ranging between 100～3000 bp． 3． Genctic distallces of 80 accessions of Cultivated types Lychee is between O．04～O．92，average O．32．Tllcy are diVided into 7 groups at the level ofthe genetic distance of0．55，Haiken 8 a11d Qiongshall 3 become印an one group alone． 4．Genetic dist锄ces o“15 accessions ofSemi．、Ⅳild Lychee is between 0．O钆0．66， average O．28．Altllough，genetic diVersity ofSemi—wildaccession Lychee in Y抽gshan area is 1a毽er，not only less tllaIl tllaf of morphOlogic，but also less也an t11at of Cultivated Cultivated types L，ychee．Meipin，Rlldao，CaIlgdao，Yngnan are Villages should be studied 111teriorly be way ofnucle盯gemlpl踮m to decide protccting or not． 5．Gen鲥c di渤nces of Cul!t：ivated types Lychee and Semi—wild Lychee is betWeen O．22～O．96 by stlldying ofme representative 34 accessions ofCumVated t)，pes Lychee 卸d