生物化学复习资料（全）-动态-Science2013.pdfVIP

Published as: Science. 2012 July 27; 337(6093): . H H M I A Absence of detectable arsenate in DNA from arsenate-grown u t h o GFAJ-1 cells r M a 1,2 3 1,4 1,5,6 Marshall Louis Reaves , Sunita Sinha , Joshua D. Rabinowitz , Leonid Kruglyak , n 3,† u and Rosemary J. Redfield s c 1Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA r i p 2 t Department of Molecular Biology, Princeton University, Princeton, NJ, USA 3Department of Zoology, University of British Columbia, Vancouver, BC, Canada 4 Department of Chemistry, Princeton University, Princeton, NJ, USA 5Howard Hughes Medical Institute, Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA H 6 H Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA M I A Abstract u t h A strain of Halomonas bacteria, GFAJ-1, has been reported to be able to use arsenate as a nutrient o r when phosphate is limiting, and to specifically incorporate arsenic into its DNA in place of M phosphorus. However, we have found that arsenate does not contribute to growth of GFAJ-1 when a n phosphate is limiting and that DNA purified from cells grown with limiting phosphate and u abundant arsenate does not exhibit the spontaneous hydrolysis expected of arsenate ester bonds. s c Furthermore, mass spectrometry showed that this DNA contains only trace amounts of free r i p arsenate and no