豌豆MAR的分离及其功能分析.pdfVIP

广西农业科学 2010 ，41（10）：1041-1045 Guangxi Agricultural Sciences ·1041 · 豌豆ＭＡＲ的分离及其功能分析 邓智年， 魏源文， 黄诚梅， 潘有强， 吕维莉， 李杨瑞* （广西作物遗传改良生物技术重点开放实验室， 南宁 ５３０００７） 摘要：从３个不同品种豌豆中分离获得３个具有ＭＡＲｓ序列特征的ＤＮＡ片段ＭＭＡＲ、ＤＭＡＲ、ＨＭＡＲ。 根据ＧｅｎＢａｎｋ同 源性搜索结果，发现这些片段均为未注册的ＤＮＡ序列。 经转化烟草并测定其ＧＵＳ活性，结果发现两侧顺式重复连接 ＭＡＲ对外源基因的表达具有明显的增强作用，ＭＭＡＲ 、ＨＭＡＲ和ＤＭＡＲ均不同程度提高了ＧＵＳ 的表达，分别为载体对 照植株的４．１６ 、３．６６和２．０８倍，但不同转化体间表达水平差异仍比较明显。 关键词：豌豆； 核基质附着区（ＭＡＲｓ ）； ＧＵＳ表达 中图分类号：S643.3 文献标识码：A 文章编号：１００２－８１６１（２０10）10－1041－０5 Isolation of pea MAR sequence and its functional analysis ＤＥＮＧ Ｚｈｉ－ｎｉａｎ ， ＷＥＩ Ｙｕａｎ－ｗｅｎ ， ＨＵＡＮＧ Ｃｈｅｎｇ－ｍｅｉ ， ＰＡＮ Ｙｏｕ－ｑｉａｎｇ ， Ｌü Ｗｅｉ－ｌｉ ， ＬＩ Ｙａｎｇ－ｒｕｉ* (Guangxi Crop Genetic Improvement and Biotechnology Key Lab, Nanning 530007, China) Abstract: Three new MAR segments (MMAR, DMAR and HMAR) were cloned from three kinds of peas. These MAR segments were different from the original MAR sequences and unregistered in GenBank. In order to investigate the gene expression of these MARs in transgenic plants, the plant expression vectors were constructed with udiA gene coding β-glu鄄 curonidase (GUS) which was flanked on both sides by the MARs in the same direction. Quantitative detection of GUS acti- vity in transformed tobacco plants showed that the MARs could increase GUS expression level in vivo in contrast to the control. The average GUS activity of the MMAR, DMAR and HMAR was 4.16, 3.66 and 2.08 times higher than the control pBI121, respectively. The expression differences among the individual transformants were also significant. ； ）； Key word