重铬酸钾鄄DNS比色法测定发酵液中乙醇含量.pdf

17 卷 1 期 生命科学研究 灾燥造援员7 晕燥援1 圆园13 年2 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Feb. 圆园13 重铬酸钾DNS 比色法测定发酵液中乙醇含量 川, 章登政, 张 俊, 杨 肖, 苏 颖, 周蓬蓬, 余龙江* （华中科技大学生命 学与技术学院生物技术系, 分子生物物理教育部重点实验室, 中国湖北 武汉430074 ） 摘 要: 发酵液中往往含有一些还原性物质, 如葡萄糖等, 用经典的重铬酸钾氧化比色法来测定发酵液中乙醇含 量时, 往往出现结果偏大的现象. 为了测定发酵液中乙醇含量, 在经典重铬酸钾氧化比色法基础上, 使用DNS 氧 化比色法测定葡萄糖含量, 修正乙醇含量的测定结果. 统计分析发现, 经改良的重铬酸钾DNS 比色法测定含葡萄 糖的乙醇标准溶液时相对误差均值为0.95%, 远小于传统的重铬酸钾氧化比色法的相对误差均值18.07%, 在精 密度相似情况 提高了准确度, 更适宜用于测定含葡萄糖等还原性成分的发酵液中乙醇含量. 关键词: 重铬酸钾DNS 比色法; 定量测定; 含糖发酵液; 乙醇含量 中图分类号: O657 文献标识码: A 文章编号 1007 7847 2013 冤01 0001 04 Potassium Dichromate DNS Colorimetric Determination of the Content of Ethanol in the Fermentation Broth HE Chuan, ZHANG Deng zheng, ZHANG Jun, YANG Xiao, SU Ying, ZHOU Peng peng, YU Long jiang* (Dep artment of Biotechnology, College of L ife Science and Technology, H uaz hong Unive rs ity of Science and Technology, Key Laboratory of Molec ular Biop hys ics, Ministry of Education, Wuhan 430074, H ubei, China) Abstract ：Because the fermentation broth often contains some reducing substances, for example glucose, when we use the classic potassium dichromate oxidation colorimetric determination of the content of ethanol in the fermentation broth, the result is often too large. In order to determine the content of ethanol in the fermentation broth, using DNS oxidation colorimetric to measure the glucose content on the basis of the clas sic potassium dichromate oxidation colorimetric can correct the ethanol content of the measured results. The statistical analysis shows the relative average error of the improved potassium dichromate DNS colorimetric measureme