基因分型技术对益生菌婴幼儿配方乳粉中双歧杆菌鉴定的研究-微生物学专业论文.docxVIP

Abstract Abstract Shanghai Normal University Master ofPhilosophy based classification technology and system evolution analysis are uesd to identify 1 2 standard strains of Bifidobacterium and 30 strains isolated from 20 probiotics powder samples． nle main results were as follows： (1)，The proportion of probiotics infant powder brand in domestic product is 36．4％，through the research and the brands official website，among 75％ofprobiotics infants COW powdered，25％of probioties baby sheep powder．In survey，the powder label that is agree with government standard，including 56％of percent of pass in domestic brand，and the percent of pass in import brands less than 50％． (2)，In the testing of the number of living Bacterium in samples，we found that 1 6 of 20 samples reaches the national standard，and the percent of pass is 90％．The number of information in the label and the number of living bacterium according、析t11 national standard is 1 3and the percent is 65％． (3)，1 6S rRNA gene sequences technology Can identify 1 2 strains standard and 30 sample strains．In the evolutionary tree，bootstrap values ofmost branches are more than 80 and has good resolution．On accout of high convervation of 16Sr RNA gene sequences，this technology Can only identifident to species level．The analysis of the samples Can be identifidented accurately． (4)，Similarity to 1 6S rRNA gene sequences classification technology,1 6S-23 S rRNA classification technology Can accurately differentiate Bifidobacterium to species level．The degree of differentiation is above 90．The identification of sample strains has hi曲resolution compared to 1 6S rRNA classification technique． (5)，MLST classification technology,Can accurately distinguish between bifidobacterium five bifidobacterium species．The degree of differentiation is 100．In addition，the technology differentiate B．animalis．ssp．1actis ATCC27673 and other B．animalis．ssp．Iactis，appraisal to intraspecific level．The technology owes high distinguishability． Key words：infant