鸡TLR3信号通路在IBDV致雏鸡法氏囊免疫损伤中的作用研究-兽医学；临床兽医学专业论文.docxVIP

CO》rn孙ITSCONTENTS CO》rn孙ITS CONTENTS Abstract in Chinese．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．1： Abstract in English．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．． ．．．．．．．．． ． ．．．．．．．．．．．．．．．． ．．．．．．．．．．．． ．．．． ．．．．． ．．．．III 1．InstructiOn 1 1．1 I沁search ofIBDV ．1 1．1．1 Gene and protein structure ofJBD．．．．．．．．． ． ．．．．．．． ．．．．．．．． ．．．．．．．．．．．．．．．．．．．．：．．． ．．．．．．．． ． ．．1 1．1．2 Seroty’pes and pathogenicity ofIBDV ．2 1．1．3 Perniciousness and prevention and ofIBD ．2 1．2 Development of chicken bursa and the ef．fect of IBDV it ．2 1．2．1 development ofbursa ofchickens ．3 1．2．2 The effect ofIBDV bursa ．．3 1．3 Research of TLR3 ． ．． ． ． ． ． ． ．．：； 1．3．1 Introduction of immune system ．3 1．：；．2 Introduction ofTLRs．．．．．．．．．．．．．．． ． ．． ．．．．．．． ．．．．．．．．． ．．．．． ．．．．．．．．．．．．．．． ．．．． ．．．．．．．． ．．4 1．：}．3 Introduction ofTLR3．．．．．．．．．． ．．．．．．． ．．． ．．．． ．．．．． ． ．．．．．．．．．．．．．．．．．．．．．．．．．． ． ．．．．．．．． ．．7 1．4 Purpose and significance ofthis study ． ． ． ． ． ．．9 2．Materials and Methods ． ． ．． ． ． ．．1() 2．1 Materials ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．． ．．．．．． ．．． ．．．．．．． ．．．．．．．． ．．．．．．．．． ．．．． ．．．．．．． ．1() ：!．1．1 Virus ． ． ． ． ． ． ． ． ． 1() 2．1．2 Experimental animal．．．．．．．．．．．．．．．． ． ．．．．． ．．． ．．．．． ．．．．．．．． ．．．．． ．．．．．．． ．．．．．． ．．．．．．．．．．．．．． 1() 2．1．3 Major chemical and biological reagents ．．10 2．1．4 Instruments ． ． ． ． ． ． ． ． ．1 1 2．1．5 Preparation ofmain reagents ．． ． ． ． ． 1 1 ：!．2 Experimental methods ．．1：1 2．2．1 Grouping ofanimals and adopting oftested materials ．．12 2．2．2 Pathological examination of IBDV．infected chickens ．．12 2．2．3 Examination of chTLR3 expression in IBDV．infected chickens ．12 2．2．4 Examination of chTLR3 signal pathway associated molecular expression in IBDV．infected chickens ．15 2．2．5 Examination of associated cytokines expression in IBDV．infected chickens ．1 5 2．2．6 Examination ofeell count ofbursa in IBDV．infected chickens 16 2．3 S