活血及利水中药对大鼠脑出血后脑水肿干预作用的研究-中西医结合临床专业论文.docxVIP

Compared with the model group，the expression of AQP4、PAR-1、TNF-α and IL-1β decreased significantly at protein and mRNA levels (P0.01)in Huoxue group and Lishui group，the brain water content showed the same result；the expression of NF-κB decreased obviously in Huoxue group，but have no difference in Lishui group ； There were also significances in statistics on the brain water content and the expression of AQP4、PAR-1、NF-κB、TNF-α and IL-1β between the Huoxue group and Lishui group； In the model group ， correlation analysis showed that there were positive correlations between the expression of AQP4 and the water content ， between the expression of AQP4 and PAR-1 and between the expression of PAR-1 and NF-κB、TNF-α and IL-1β respectivedly； Significant pathological changes were happened around perihematoma，while they were improved in Huoxue group and Lishui group respectivedly. Conclusions The road of Thromb-PAR-1—inflammation reaction perhaps a part of upstream signaling pathways of AQP4；that is to say，probably through the inflammation reaction mediated by Thromb-PAR-1 ， the expression of AQP4 around perihematoma was upregulated，this resulted the edema of ICH； Huoxue Herbs and Lishui Herbs can decrease the edema of ICH，the probablely mechanism was that it could regulate the expression of related proteins and cytokines around perihematoma，but herbs of different functions have different effects ,targets sand intensity. Key words intracerebral hemorrhage；brain edema；blood stasis；Huoxue herbs； Lishui herbs. 目 录 引 言 1 第一部分 理论研究 3 一、出血性中风血瘀病机的中医学研究 3 （一）血瘀理论 3 （二）出血性中风血瘀病机的确立 5 （三）出血性中风血瘀病机的形成及其演变 7 （四）活血化瘀法在出血性中风中的应用 7 二、脑出血后脑水肿的西医学研究 9 （一）脑出血后脑水肿的分子生物学基础研究 10 （二）脑出血后脑水肿的西医治疗研究现状 17 第二部分 实验研究 22 一、主要实验材料和仪器 22 （一）仪器 22 （二）试剂 23 （三）主要溶液的配制 24 二、实验方法 27 （一）实验动物及分组 27 （二）动物模型的建立 27 （三）实验药物的制备及用药方法 27 （四）标本的取材和制备 28 （五）神经功能缺损评分 28 （六）大鼠脑组织含水量的测定 29 （七）HE 染色流程 29 （八）免疫组化法检测 AQP4、PAR-1、NF-κBp65、TNF-α及 IL-1β的表达 29 （九）Real-time PCR 检测脑组织 AQP4、PAR-1、NF-κBp65、TNF-