加压毛细管电色谱基本理论研究及其在手性药物分离中的应用-有机化学专业论文.docxVIP

摘要f加压毛细管电色谱是毛细管电色谱的一种新型分离模式，它采用电渗流和压 摘要 f加压毛细管电色谱是毛细管电色谱的一种新型分离模式，它采用电渗流和压 力流来共同驱动流动相，基本上解决了气泡问题，增加了毛细管电色谱方法的 稳定性。4 ／ 首次采用新型的专用毛细管电色谱仪TriSepTM．2000GV系统对加压毛细管电 色谱的基本理论及其在手性药物分离方面的应用进行了研究。 以中性、碱性、酸性共六种物质作为考察对象，研究了加压毛细管电色谱的 保留机理。通过建立一个数学方程来描述中性、碱性、酸性物质的容量因子k’ 与电场强度、压力流速、电渗淌度和电泳淌度的关系，并将理论值和实验值进 行了对比研究。 选取具有代表性的十五种手性药物进行了P—CEC和1．t．HPLC的对映体分离 对比研究：以异丙嗪作为考察对象，研究了电场强度、压力和流动相对手性分 离的影响，逐步优化从而确定对映体分离的优化条件，并将前面建立的数学方 程应用于手性分离。 关键】；莸 加压毛细管电色谱j 毛细管电色谱， 基本理啄 手性药物? 对映体分离 万古霉素： AbstractPressuried Abstract Pressuried Capillary Electrochromatography(P—CEC)is a novel chromatographic technique，in which the mobile phase is driven by eletroosmotic flow and pressuried flow．P-CEC call prevent bubble formation and provide stability and reproducibility of the chromatographic performace．therefore one of the major drawbacks of capillary electrochromatography solely driven by eletroosmotie flow is diminished． Research on basic theory and enantioseparation in P-CEC was tested On TriSepTM- 2000GV pressuried CEC system． With neutral，basic，acidic solutes as model solutes，retention behavior of P—CEC was investigated．A mathematical model describing the relationship of capacity factor with electrical field strength，pressurized flow velocity,electroosmotic mobility,and electrophoretic mobility is estabished，which is used to simulate and explain the experimental data． The enantioseparation of 15 chiral pharmaceuticals，which have different pharmaceutical activities and are difficult to separate were investigated on both P- CEC and p．HPLC with vancomycin as chiral stationary phase．With pmmethazine as model solute，the effects of electrical field strength，pressure，and mobile phase were investigated． Key word：pressuried capillary electrochromatography capillary electrochromatography basic theory chiral pharmaceuticals enantioseparation vaneomyein ————2旦墼量塑』型翌主生些堡塞 ————2旦墼量塑』型翌主生些堡塞 !!!!生!旦 第一章前言 一、 毛细管电色谱简介 (一)基本特点 毛细管电色谱(Capillary Electrochromatography，CEC)是近十年来综合了 现代最新分离技术高效液相色谱(High Performance Liquid Ch