加味茵陈蒿汤对急性肝损伤大鼠肝组织TLR4信号转导通路及Th1Th2影响的实验研究-中西医结合临床专业论文.docx

- - PAGE 10 - 结论 1. 加味茵陈蒿汤低、中、高剂量治疗组均能显著减轻D-氨基半乳糖所致大 鼠的肝细胞损伤，降低血浆中ALT、AST、TB含量，具有较好的保肝降酶作用。同 时在病理方面，均能显著改善肝组织的病理变化，缩小病变的面积，减轻肝细胞 的损伤程度。 2. 加味茵陈蒿汤低、中、高剂量治疗组均能显著减轻D-氨基半乳糖所致大 鼠的肝细胞损伤，通过调节Th1、Th2类细胞因子，纠正Th1/Th2的免疫失衡状态， 调节机体的免疫反应而起到保肝作用。 3.加味茵陈蒿汤高剂量和中剂量治疗组对D-氨基半乳糖所致急性肝损伤大 鼠的TLR4信号转导通路均有一定的影响，可能通过调节细胞因子-细胞信号转导 通路起到保肝的作用。 关键词：加味茵陈蒿汤、急性肝损伤大鼠、Th1/Th2、TLR4 信号转导通路 本课题受以下项目资助：国家中医药管理局中医重点临床研究中医肝胆学， 项目编号：CJX2010001，四川省科技厅基金项目资助，项目编号：2011SZ0015。 Abstract Objective: To research the mechanisms of supplemented Yin-Chen-Hao decotion representing the methods of detoxification and removing dampness , nourishing yin and yi qi that affects TLR4 signal transduction of the rats liver tissue and Th1/Th2 for acute liver injury of rats induced by D-GalN. Method: 72 female SD rats of clean class were randomized into six groups with 12 in each group: blank control group, model group, Compound Glycyrrhizin Tablets group, and low, middle and high dosage group of supplemented Yin-Chen-Hao decotion. All the rats were fed adaptedly with common feed and clean water in the 15-20degrees and humidity level at 60-80% of laboratory for seven days, seven days later, single intraperitoneal injection of D-GalN at a dosage of 600mg/kg was administered to the rats except those in the blank control group to induce acute liver injury. Medications were given orally to each treatment group at the same time, of which the daily dose of Compound Glycyrrhizin Tablets was 15.75mg/kg, and dosage of the three herbal groups were 14.7g/kg，29.4g/kg and 58.8g/kg respectively. The blank control group was fed with the 0.9% physiological saline .All the medications were given at a volume of 10ml/kg, twice a day for two days. 48 hours later, all the rats were sacrificed with their serum ALT, AST and TB being examined. The expression of TNF-α，MIP-2，IL-2， IL-4，IL-10 and IFN-γ were analyzed with the enzyme-linked immuno sorbent assay. Immunohistochemical method is used to measure the content of MyD88、TRAF6、IkB-α、 P38MAPK and TLR4