基质力学调控肿瘤细胞靶向捕获和粘附行为的血流动力学研究-细胞生物学专业论文.docxVIP

重庆大学硕士学位 重庆大学硕士学位论文 英文摘要 PAGE PAGE VI ABSTRACT Tumor metastasis, a main biological characteristic of malignance tumor, is an important cause of death for most cancer patients. During the tumor metastasis, the arrest and adhesion of tumor cells within microvessels is not only an initial step of tumor metastasis in blood vessel, but also a vital process for the organ-specific metastasis. Recent research results showed that, the tumor cells in blood circulation are captured in capillary. But, the action of tumor cell adhesion is not random, it exists HYPERLINK /s?wd=prominent prominent organ discrepancy (named as organ specificity of tumor metastasis). The most common target organs are lung for breast cancer metastases, followed by liver, bone, and it is rarely found in other organs. However, the organ-specific mechanism of tumor cells firstly adhered to microvessels, which is important for studying the organ-specific metastasis, still remains elusive. By using the parallel plate flow chamber to simulate the blood flow in vivo environment, this paper explores the effect of matrix mechanics on tumor cell arrest and adhesion in specific target organ, as well as analyze the correlation between the expression of integrin β 1 and adhesion behavior. The results will contributes to understand and cognize the effect and mechanism of matrix HYPERLINK /s?wd=mechanical%20property%20 mechanical properties of distinct organ on tumor cell arrest and adhesion in blood vessel, and provide a theoretical foundation for treating cancer disease, as well as developing new drugs for tumor metastasis. Here, the concrete works and conclusions are as the following: Object: By analyzing and comparing the effect of hemodynamics and matrix rigidity on the tumor cell arrest and adhesion behaviors with / without endothelial cells and chemokines, this paper will discuses 1) Whether matrix rigidity is involved in the regulation of organ-specific tumor cell arrest and adhesion or not, and what is