急性重症胰腺炎大鼠模型脑组织中md2表达及其与炎症相关性-内科学(消化内科)专业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 3 3 The expression and inflammatory correlation of md2 in the brain of rats after inducing severe acute pancreatitis Abstract Objective: To explore the expression and inflammatory correlation of md2 in the pathphysiology of pancreatic encephalopathy during severe acute pancreatitis. Methods: 64SD rats were randomly divided into SAP group and Control group.SAP model was induced by retrograde bile-pancreatic duct infusion of 5% sodium taurocholate(NaTc).The control group was infused 0.9% Sodium chloride by the same methed. In all rats, the pancreatic pathology, the permeabilityof BBB, the expression levels of md2 and TNF-α in brain were observed at 2, 6, 12, 24h after operation. Results: Compared with the control groups, the pancreatic tissue in SAP groups presented a lot of edema ,hemorrhage,necrosis and inflammatory infiltrate cells.The expression level of md2 protein and md2mRNA showed remarkbly up-regulation in SAP groups(p0.05).The level of TNF-αmRNA showed a correlation with md2mRNA at 2,6, 12,24h. The permeability of BBB showed remarkably increase at all time points (p0.05). Conclusions: The SAP models were induced successfully by retrograde bilepancreatic duct infusion of 5% sodium taurocholate(NaTc). Md2 exists in normal cortex tissue and expresses remarkbly upregulation in the SAP groups. There were correlation between the permeability of BBB ,the expression levels of TNF-αmRNA and the expression levels of md2-mRNA .The express of md2 protein possibly participates in inflammatory signaling pathways of SAP, and leads to the increase of permeability of BBB and the release of inflammatory cytokins. 【Key words】 pancreatitis; blood brain barrier; md2; TNF-α; permeability. 1 1 英文缩略表 英文缩写 SAP PE MD2 TLR4 LPS LBP MOF BBB TNF-α MyD-88 英文全称 Severe acute pancreatitis pancreatic encephalopathy Myeloid differentiation protein –2 Toll like receptor-4 Lipopolysaccharide Lipopolysaccharide Binding Protein Multiple organ failure Blood br