家禽养殖场废弃物农用安全性的初步研究-植物营养专业论文.docxVIP

ompA、加和luxs在所有的65株大肠杆菌中均存在，是大肠杆菌的保守基因。同 ompA、加和luxs在所有的65株大肠杆菌中均存在，是大肠杆菌的保守基因。同 时证实了APEC的优势基因型是：iss*iuclYtsh+cvaCiroCfimCompA+pfs+1uxS*基因 组合模式。(5)luxS／AI一2型密度感应系统参与调控细菌众多的生理功能，细菌 的1uxS基因参与信号分子AI一2合成。通过哈维弧菌(Vibrio harveyi)BBl70对 大肠杆菌的AI-2信号分子进行检测，大肠杆菌可以产生AI一2信号分子。并且发 现，AI-2的产生是生长依赖性的，在对数生长期后期大肠杆菌产生A工一2的量最 大。 综上所述，本研究通过对禽场环境中的大肠杆菌的生长特征、耐药性、血 清型、毒力基因分布及信号分子AI一2的研究，明确家禽养殖场废弃物直接利用 极不安全，在我国目前的家禽养殖生产条件下，必须对废弃物进行无害化处理 后才能加以利用。 关键词：大肠杆菌分离鉴定；耐药性；血清型；毒力基因分布； AI-2检测 AbstractThe Abstract The utilization of poultry farm waste is more and more widely recognized． However,the security of agricultural waste from the poultry farm Was lack system study,especially the ecological characteristics of E．coli in poultry farm．Escherichia coli(E．coli)is widespreading in environments，which are prokaryotes and Gram—negative．Some of its specific serotypes can cause human and animal severe diarrhea and sepsis．E．coli can pollute water and soil by human and animal feces or by organic fertilizer and sewage，thus widely in the biosphere． As a representative type of environmental microbiology,Escherichia coli existence detection has been widely used in commercial organic fertilizer,organic fertilizer and environmental pollution．In bacteriological examination of environmental pollution, the coliform bacteria detection are indicators of fecal contamination and the number of coliform bacteria is determine the level of faecal pollution degree and the dangers sizes to human and poultry health．There study the ecological characteristics of E． coli and its affecting the safety of farm waste utilization． The main contents include：(1)study the number of E．coli presence and proliferation of dynamic in poultry farm；(2)analysis the popular serotypes of E．coli， 65 E coli isolated from the typical colibacillosis lesions of illness and death poultry by positive serum for glass plate agglutination test；(3)study E。coli resistance spectrum and resistance patterns using antibiotic susceptibility test；(4)Survey the mol