激光和高压静电场克服植物自交不亲和性和远缘杂交不亲和性的分析-园林植物与观赏园艺专业论文.docxVIP

摘 摘 要 本研究足以羽农j|蓝(Brassica oleracea vat，acephala)和草原她胆 (Eustomagrand!／ttinl)的自交不亲和系，及黑芥(Brassica nigra)翱窟 菜(Brassica campextt+is)为试耱。餐精激光秘裹嚣黪魄场怼试耱豹花粉逐 行处理，以期克服{’l交不亲和悭和远缘杂交不亲和性。通过对处理厝的花 粉的表面结构和蛋|，l慨活性、及麒在柱头上的萌发情况的研究，进一步分 析激光和高压静电场的生物效应。利用Northern杂交技术和双向电泳技 术，检测了经激必承I高垂静电场娥理豹藐粉，在授耪簸棱头上SLG翻SRK 基因表达的差异移蛋自质图谱懿差异，以探讨激光秘菇压静毫场对蘩殖反 应的作用机制。通过|．-述实验褥到如下结论： 1、通过适宜ii：【j辩}的激光和高压静电场处理，羽农甘蓝和草原龙胆的 花粉萌发率提麓，{’l变亲和指数和结籽率提高：同时自交后代植株的缴长 势氇有较丈段落，幼茁毽壮，测定植绦的生物量也最萋提高。 2、献花粉的表嘛结构的电镜显微观察分桥，遴遥激光和离歪静电场 处理后的花粉，其花粉壁的雕纹受到了伤害，网脊线有些断裂，网眼交大， 花粉管原始体炎长，并且成熟花麴的水溶性总蛋白，n一淀粉酶活力，总 淀粉酶活力均不潮程度的提高。 3、经激光稻离豫势电殇楚疆的花羧自交嚣，酸不弱时淘阗隔懿疆头 样品提取总RNA，利用Northern杂交技术对SLG和SRK基因的表达蛩谶行 检测。结果表明，授粉后8小时，激光和高压静电场处理与对照之间SLG 和SRK基因的袭达挝见明显差异。授粉后24小时激光处理的SLG和SRK 基因的表达量比对照嘲显降低，但高压静电场处理与对照无疆曼差异。 4、未授粉疆头懿蛋鑫震点鞠遐多子援耱盖较头瓣蛋白囊煮{授羧爱 一些碱性端的蛋自点消失，同时一+些小分子量的蛋白点减少。用激光处理 后的花粉与对照花粉住授粉后8和24小时进行比较，在处理与对照之间 找到了特异蛋白点。 关键词：激巍、蕊堰静电场、爨交不亲和牲、运缘杂交不亲秘性、筏耪 AbstractIncompatible Abstract Incompatible cultiwlrs of Brassica oleracea var．acephala and Eustoma grandiflorum．and b i of Brassica nigra and Brassica campestris were used in this experiment The pollens of each plant were treated by laser and high·voltage electrostatic field for overcoming their self-incompatibility and cross。incompatibility．The biological effect of laser and high-voltage electrostatic field the treated poIlens was studied further by analyzing the surface structure，proteinase activity and germination the stigma In addition，expression difference of SLG and SRK genes and spectrum difference of protei of stigma after pollination of the treated pollen were analyzed by Northern blotting and protein 2-D electrophoresis．The mechanism involved in the effect of laser and high—voltage electrostatic field reproductive process was discussed．The results follows． 1．At appropriate dosage of laser and high—voltage electrostatic field，the germination of pol len in Brassica oleracea var．acephala and Eustoma grandiflorum was inct eased，the self-compatibility index and the number of seeds were increased．a