霍山石斛多糖提取分离以及抗肿瘤活性的研究-食品科学专业论文.docxVIP

Extraction，separation Extraction，separation and anti-tumor activity of poIysaccharides in Dendrobium huoshanense Abstract Dendrobium huoshanense is the earliest recorded Medicinal plant in China，and is famous for its medicinal value．Polysaecharide is one of the most important active ingredients in it．In this paper，the process of extracting polysaccharides form Dendrobium huoshanense is optimized，and several polysaccharide components are separated．Afterwards，anti-tumor activity of polysaccharides is researched in human gastric cancer cell SGC-7901．It primely explored the effect of polysaccharide on the tumor—associated genes， p53 and e-mye． With single factor experimental design，Box-Behnken experimental design and response surface analysis，the mathematical model of Dendrobium huoshanense polysaecharide extraction rate was established based on data of extraction temperature，pH and extraction time．Equation of the model is Y=O．175767+0．023363X1+0．013662X2·0．032858X12+O．019XlX2·0．026308X22． The optimum conditions were temperature of 69．4C，time of 1．57h，pH of 5．9， ratio of material to water of 1：20 and 2 times CXtraction．Under these conditions．the model value was coincident with the actual value by 98．4％． Comparison of two deprotcinization methods and five decolorization methods suggest，although deproteinization rate of Sevag method was Less than that of TCA method，the polysaccharide content was maintained better． Sevag method was a better deprOteinization method．Decolorization with DEAE·cellulose had a higher decolorization rate，less loss rate．Although DEAE·cellulose is expensive，its recycling is convenient．DEAE—cellulose is a better mateiraI to decOlorization． Dendrobium huoshanense polysaccharidc extracted with hot water was separated by DEAE—cellulose ion exchange chromatography．POlysaccharidc HDP-1，HDP一2，HDP一3，HDP，HDP-5，HDP一6 were gained．The polysaccharide HDP··2 with maximum anti··tumor rate was continuously separated by Sephacryl·200HR chromatography．Two p01ys