马陆共生放线菌与其抗真菌活性物质的分离鉴定.pdfVIP

CONrENTS CoNTENTS inChinese………………………………………………………………………………………………………．I Abstract Abstractin English……………………………………………………………………………………………………．．III 1Introduction………………………．……………………………………………………………………………………．．1 1．1Thecurrentsituationof of disease……………………………………………1 fungi preventionplant 1．2 methodofthemicrobialantibiotics……………………………………………………………．1 Screening 1．3Metabolitesresearchofinsects actinomycetes………………………………………………2 symbiotic 1．4 Millipede………………………………………………………………………………………………………………4 1．5 and ofthe study……………………………………………………………………．．5 Objectivesignificance 1．5．1 Significance…………………．…………………………………………………………………………………5 1．5．2 Objective………………………………………………………………………………………………………．．5 1．6Researchsource．……………………………………………………………………………………………………．6 2MaterialsandMethods………………………………………………………………………………………………．8 2．1Materials………………………………………………………………………………………………………………8 2．1．1Samples…………………………………………………………………………………………………………．8 2．1．2Test fungi………………………………………………………………………………………………………．8 2．2 and ReagentsEquipments………………………………………………………………………………………．．8 2．2．1 Reagents…．……………………………………………………………………………………………………．．8 2．2．2 Equipments……………………………………………………………………………………………………．．8 2．3Mainmedia…………………………………………………………………………………………………………．．9 2．3．1Isolationmedium……………………………………………………………………………………………．．9 2．3．2Purifiedmedium……………………………………………………………………………………………．10 2．3．3 medium……………………………………………………………………………………………．10 Mycelia 2．3．4 medium…………………………．………………………………………………………………．．10 Bioassay 2．3．5 andbiochemicaltextmedium………………………………………………………．．10 Physiological