外源一氧化氮对干旱胁迫下白花蛇舌草种子萌发的影响.PDF

·840 · 中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 6 期 2014 年 3 月 外源一氧化氮对干旱胁迫下白花蛇舌草种子萌发的影响 * 朱再标，宁梓君，郭巧生 ，曹亚悦 南京农业大学 中药材研究所，江苏 南京 210095 摘 要：目的 探索外源一氧化氮（NO ）对干旱胁迫下白花蛇舌草Oldenlandia diffusa 种子萌发的影响，并确定缓解干旱胁 迫的硝普钠（SNP，NO 外源供体）最适浓度。方法 用 15% PEG 6000 模拟干旱胁迫，测定不同浓度梯度 SNP 处理后干旱 胁迫下白花蛇舌草种子的发芽势、发芽率、发芽指数和活力指数。结果 干旱胁迫显著抑制了白花蛇舌草种子的萌发过程。 SNP 能显著促进干旱胁迫下白花蛇舌草种子的萌发，0.3 mmol/L SNP 处理可提高发芽率和活力指数 2.78 倍和 7.77 倍；从发 芽势和发芽指数来看，0.1 mmol/L 和 0.3 mmol/L SNP 处理效果最佳且二者差异不显著。结论 SNP 可显著缓解干旱胁迫对 白花蛇舌草种子萌发的不利影响，提高白花蛇舌草种子的抗旱能力，其中以0.3 mmol/L SNP 效果最佳。 关键词：白花蛇舌草；种子萌发；干旱胁迫；一氧化氮；硝普钠 中图分类号：R282.21 文献标志码：A 文章编号：0253 - 2670(2014)06 - 0840 - 04 DOI: 10.7501/j.issn.0253-2670.2014.06.019 Effect of exogenous nitric oxide on seed germination of Oldenlandia diffusa under drought stress ZHU Zai-biao, NING Zi-jun, GUO Qiao-sheng, CAO Ya-yue Institute of Medicinal Plants, Nanjing Agricultural University, Nanjing 210095, China Abstract: Objective To explore the effect of exogenous nitric oxide (NO) on seed germination of Oldenlandia diffusa under drought stress and to determine the optimal concentration of sodium nitroprusside (SNP, exterior nitric oxide donor) for alleviating the drought stress. Methods Using 15% PEG6000 to imitate drought stress, the germination potential, germination rate, germination index, and vigor index of O. diffusa seeds were measured by the treatment of SNP at different concentration. Results The germination process of O. diffusa seeds was significantly suppressed by drought stress. SNP could significantly promote the germination of O. diffusa seeds under drought stress. SNP (0.3 mmol/L) increased the germination rate and germination potential by 2.78- and 7.77-folds, respectively. In the case of germination index and vigor index, SNP (0.1 and 0.3 mmol/L ) showed the maximum positive impact and there w