鸡2号染色体短臂连锁图谱构建与玫瑰冠性状的连锁分析-动物遗传育种与繁殖专业论文.docxVIP

II II Abstract In this study, three Tulufan fighting cocks and six rose comb hens both with the homozygous comb genotype were chosen as the parents through test cross.Using F2 and backcross experimental design, we cumulatively collected some individuals with comb trait record. Among the individuals, F1 generation had twenty-eight individuals (all walnut comb), of which six were cock, twenty-two were hen; F2 generation had seven hundred and thirteen individuals, including four hundred and three walnut comb individuals, one hundred and forty-seven rose comb individuals, one hundred and nineteen pea comb individuals, and only forty-four single comb individuals; backcross population had sixty-seven individuals, of which thirty-two were pea comb individuals, thirty-five were walnut comb individuals. By the chi square test, the proportion of four combs of the F2 generation was accorded with 9:3:3:1, and the backcross population’s comb proportion was in line with 1:1, which were both consistent with the discussion of classical genetics. Based on the published genetic linkage map of chicken, seventeen microsatellite markers were selected in the chicken’s short arm of chromosome two(ADL0228、ABR0336、 ABR0020、ABR0004、 MCW0082、MCW0341、ADL0270、LEI0320、LEI0117、MCW0247、ADL0309、MCW0131、LEI0086、 MCW0239、ADL0120、MCW0065、ADL0257) for the genetic structure analysis of experimental population. Meanwhile, we used Crimap2.4 and MapDraw2.1 software to build the genetic linkage map of this group’s short arm of chromosome two, and combined with phenotypic records to analyse the mapping of comb trait, the results were as follows: 1、The seventeen microsatellite loci all showed higher heterozygosity and polymorphic information content in the experimental population. The average population heterozygosity was 0.6900 (0.3396-0.9196), and the polymorphic information content was between 0.3003 and 0.7017. It can be seen that the seventeen loci were moderate or high polymorph