慢性应激所致抑郁大鼠脑内促炎性细胞因子及脑白质的变化精神病与精神卫生学专业毕业论文.docxVIP

汕头 汕头大学医学院硕士学位论文 PAGE PAGE III 1. 慢性不可预测性温和应激 28 天可诱导大鼠抑郁样行为； 2. 抑郁样行为伴随着脑内促炎性细胞因子 IL-6?IL-1β和 TNF-α的表达水平升高，提示 二者相关； 3. 抑郁样行为伴随着前额叶少突胶质细胞数量减少和 MBP 样免疫反应性降低，提示二者 相关； 根据结果推测：慢性应激通过影响脑对神经内分泌和免疫系统的功能调节提高脑内促 炎性细胞因子 IL-6?IL-1β和 TNF-α的表达，后者损害某些脑区的少突胶质细胞进而影 响脑的高级功能活动?由此，我们的实验结果为慢性应激大鼠表现出的抑郁样行为提供了 一种合理的解释? 关键词：抑郁症；慢性应激；促炎性细胞因子；脑白质；少突胶质细胞 PAGE PAGE IV Abstract Background and objectives The pathogenesis of depression, one of major mental disorders, still remains to be elusive. In addition to the traditional theories, such as the monoamine hypothesis, proinflammatory cytokines and white matter lesions have been suspected to be important contributors to the onset and development of depression in some studies, with the following supporting evidence: 1) patients with major depressive disorder (MDD) showed higher levels of some inflammatory molecules; 2) patients with inflammation in either CNS or peripheral tissues are associated with higher morbidities of major depression; and 3) patients treated with cytokines for various illnesses are at increased risk of developing MDD. Depressive patients, examined by the diffusive tensor imaging (DTI) in clinical studies, showed white matter lesions at various extents. The aim of this study was to examine the relationship of some proinflammatory cytokines and white matter lesion in the brain and depression-like behaviors in rats. Materials and methods Adult male Sprague-Dawley (S-D) rats weighting 220-270 grams were used. The S-D rats were divided into two groups randomly: the normal control group (n = 8) and the stressed group (n=8). Rats in the stressed group were were feed separately,and subjected to chronic unpredictable mild stress (CUMS) for 28 days, while normal controls were raised four per cage in the same animal center without CUMS. Sucrose preference test, open field test, and forced swimming test were performed to measure the behaviors of both normal controls and stressed rats. Luxol Fast Blue-Periodic Acid Schiff sta