抗草甘膦大豆种质挖掘及抗性机制研究-植物保护；农药学专业毕业论文.docxVIP

o∞订匿N．rSCoNTENTSAbstract姬Cmnese．．1Abstract in English：．】【II 1 Introduction．．．．．．． ．．．．．．1 1．1 Soybean development situation and status ofweeds in soybean fields in Chinese and abroadl1．1．1 Developmentstatusofthe soybeanindomestic．．j11．1．2 Development ofSoybean overse自Lq．．．．．21．1．3 The oocun℃nce ofweeds in soybean fields and Control．．4 1．2 Mechanism ofaction ofglyphosate andresearch status．．．．．．。．．．4 1．2．1 Glyphosate introduction．．．．。．。．．．。 。41．2．2 Mechanism ofglyphosate． ．．．．．．．．，5 1．2．3 Mechanism of Glyphosate Resistance ．．．．．．．．-．61．3 Methods ofglyphosate resistant resources excavation methods：一71．3．1 Methodsofobtainedglyphosate resistant resourcesbymeans ofGMtechnology ．7 1．3．2 Methods of obtained by glyphosate resistant resources by means of non二genetically modifled ．j ．81．3．3 Screeningofglyphosate resistantsoybean resouices ．．． ．．．．．．． ．II 1．4 Current Situation ofnon-transgenic herbicide-resistant crops ．1 1 1．5 Purpose and significance ofthis study．．．． 。 ．．。．。。． ．．．． ．． ．121．6 Technical roadmap ofthis study．．．．．．．．．．．．．．．． ．．．．13 2 Materials and methods． ．．．．．．．．．．．．． ．．．．．．．．． ．．．．14 2．1 Experimental Materials ．．．．．． ． ．．．．．． ．．14 2．1．1 plant material”142．1．2 Common reagents．!142．1．3 Main instrument equipment一142．1，4 Field screening test。·142j2 Method．142．2．1 Different soybean varieties resistant to glyphosate ． ．． ．． ．．-．14 2．2．2 Genetic stability ofglyphosate-resistant soybean ． ． ”14 2．2．3 Bioassay ofdifferent soybean varieties ． ． ． ．．142．2．4 Roundup Ready Soybean Physiological and Biochemical Mechanism．．1 52．3 Data analysis．． ．． ． ．．．．． ． ． ． ．．．． ． ．．．． ．．． ． ．． ．．． ．．．．． ．．．．17 3 Results and analysis．．．． ．． ．．．． ．．．．．． ．． ．．． ．．18 3．1 Roundup Ready Soybean Germplasm Screening183．1．1 Resistance ofdifferent soybean varieties to glyphosate．18 3．2 Establishment ofRoundup Ready soybean bioassay method ．．．．．．． ．·44 3．2．1 Glyphosate on soybean growth process in different parts of the Growth Glyphosate onIll万方数据_l东北农业大学农学硕士学位论文、。．．．_—■■■■_■-_