方法和组分的分析用于增强经皮给药.docVIP

Methods and compostions for enhancing transdermal drug delivery US 20090291133 A1 Methods and compositions for enha ncing tran sdermal delivery of a bioactive age nt. The method contains the step of applying to a skin tissue an effective amount of a composition comprisi ng: (a) a drug vehicle; (b) a bioactive age nt en capsulated within the drug vehicle; (c) a plurality of proteolytic enzyme molecules conjugated onto the surface of the drug vehicle; and (d) a pharmaceutically acceptable carrier, for a period of time effective to deliver the bioactive age nt across the skin tissue at a desired dosage ? 图片(3) 权利要求(20) A method for enhancing delivery of a bioactive age nt across a skin tissue comprisi ng the step of: applying an effective amount of a composition comprising: a drug vehicle; a bioactive age nt en capsulated within the drug vehicle; a plurality of proteolytic enzyme molecules conjugated onto the surface of the drug vehicle; and a pharmaceutically acceptable carrier, for a period of time effective to deliver the bioactive age nt across the skin tissue at a desired dosage. The method of claim I, wherein the drug vehicle is a liposome? The method of claim 2, wherei n the composition further comprises a nano particle en closed in the liposome, wherein the bioactive age nt is en capsulated within the nan oparicle within the liposome. The method of claim 3, wherein the nan oparticle is formed from poly(lactic-co-glycolic acid) (PLGA) or hydro-gel. The method of claim 1, wherein the proteolytic enzyme is papain. The method of claim 5, wherein the concentration of papain is less than 93, or less than 50, 40 or 30 pg/ml. The method of claim 1, wherein the proteolytic enzyme is a selected from the group consisting of papain, pancreatin, ficin, bromelain, elastase, pepsin, hyaluronidase, streptokinase, streptodomase, trypsin, chymotrypsin, a-chymotrypsin, a-amylase, deoxyrib on ucLIease, collage nase, sutilai n and any combi nation thereof. The method of claim 1, wh