棉花细胞质雄性不育系线粒体基因组细菌人工染色体文库的构建及其鉴定-分子生物学与生物化学专业毕业论文.docxVIP

AbstractThe Abstract The colton is one of the majn industfjal cropS in ouf counlrH Wilh Ibe fextile industry and the Jjves of the people 1evel enhancement，China alTeady become the biggest cotto“producer country and elpense country in the worId．Wilh incre船ing unceasin91y t0 cotcon毫demand，m order to meet people、口eed，出e cullivation of hi91l production，the high quality cotton new Variety to s01Ve t11e question is她incvitable way Now，t11e world cotton brceding main research direction usillg the cotton cytoplasmic m“e—slerile line cuIlivat抽n co【ton hybrid var主ecy．The massive research indicate that oytoplasmic male sterility has 111e close relation wi也tlle mitochondr瑚DNA variation．Therefore，in order to fesearch oytoplasmic male sterility wifh mjtochond“al DNA between reJations furfheL出is rese3rch wili cons￡『uct cofc()n mifo曲ondfial DNA bacterial an琦cial chromosome 1mrary，and has carricd on the preliminary appraisal to the lib豫ry，male sterility bunds the solid founda【ion fof more thorougb understandi“g and cxplana60n cytopIa5m w“h Ihe mitochonddon between relations． This陀search toke tlle upland cotton(Gosl)pf啪^批，Hmm)cytophsmic male·sterile line P30A e￡joJa￡ion seed】jng as the脚ate“aj．Refe佗nce nce，maize and oth盯cfops’Jl】nocbondrial DNA bactedal anificial chromosome librafy constmction methods，加d uⅡifies cotton itself lo indude me gossipol，tannin easny to ox埘jzc materiaIs’parlicularity，estabJished a h远h quaIj‘y mitochondfiaJ DNA’s BAc librafy system．nis sysI锄contained three very important condjtions：the high molecular weighI mtDNA exIraction，dephosphorizationeffectgoodcarriefpreparation，transfonnede茌iciencyh嘞electroco叩ten cell preparation． 1n th。process of s8parating mitochoⅡ“on and its DNA，fhe mjtochondrjon which曲taiⅡed using discontinuous sucmsc gradjen￡cenfr，fugation，afler曲e玎uoroscope examination jndjcafed fhafics jntc鲥￡y was good，did not have ponutions such as chloroplasI and ceU nucleus and so on．We obta．med mi【ochondrial DNA using the embedding decomposition method．And e