黄芪多糖小肠吸收机制研究-药剂学专业毕业论文.docxVIP

中文摘要黄芪多糖是中药黄芪的主要成分之一，研究证明黄芪多糖具有免疫调节、抗 中文摘要 黄芪多糖是中药黄芪的主要成分之一，研究证明黄芪多糖具有免疫调节、抗 肿瘤、抗病毒，保护心脑血管功能，抗衰老和调节机体代谢等广泛的药理作用， 大量的黄芪多糖口服制剂已经被应用于临床。但是，关于黄芪多糖的胃肠道吸收 机制的研究还是空白，本文采用大鼠外翻肠囊法考察影响黄芪多糖吸收的多种因 素，探讨黄芪多糖的小肠吸收机制。 本文采用大鼠外翻肠囊法，考察了吸收部位、pH值，给药浓度对黄芪多糖 小肠吸收率的影响。实验结果表明：不同肠段部位(十二指肠，空肠，结肠)间 药物吸收无显著差异(p0．05)，缓冲液的pH值对药物吸收有影响(p0．05)， 其中在p8为6．78时吸收最好，单位时间吸收的药量与浓度不成正比例关系，有 饱和现象。结果提示黄芪多糖是通过小肠上皮细胞转运的。考察了不同吸收促进 剂对药物吸收的影响，结果表明牛黄胆酸钠、十二烷基酸钠和吐温80对黄芪多 糖均有较为明显的吸收促进作用。 实验过程采用葸酮．硫酸法测定黄芪多糖含量，3，5．二硝基水杨酸法测定粗 品中还原糖含量，这些方法线性范围良好，回收率较高，方法准确可行。此外还 对所购买的黄芪多糖粗品进行了脱蛋白、脱色等提纯处理，发现III型ZTCI+I天 然澄清剂的脱蛋白效果优于Scvege法，双氧水脱色的效果略低于D280阴离子大 孔吸附树脂，经提纯后黄芪多糖纯度达到69．9％，基本除净了还原糖和蛋白质。 关键词：黄芪多糖外翻肠囊法蒽酮．硫酸法吸收机制 ABSTRACTAstragalus ABSTRACT Astragalus polysaccharide(APS)is the main component of Astragalus mongholicus，a well-known naturceutical in Chinese traditional medicine．Astragalus polysaccharide seem to be able to stimulate white blood cell production and spurs the activity of killer T cells，thus strengthen the ilRmune system．The preparation now available on the manet includes injection、oral solution and granules．However,the mechanism of intestinal absorption for Astragalus polysaccharide is not yet clear． Rat evered gut sac system of different intestinal section(jejunum，ileum，and duodenum)was used for the study on absorption characteristics and the absorption mechanism of APS．The solution inside the sac was collected and the APS concentration was determined by anthrone—sulfuric acid colorimetry．The data showed that APS absorption rate was the same for all sections of the intestine(pO．05)，but varied in buffer solution with different pH values．Best absorption rate was observed when buffer solution had a pH value is 6．8．The absorption rate did not show a linear relationship with the increment of dose or concentration，suggesting a phenomenon of saturation for the absorption．Therefore a transpoter may be needed for APS absorption．The effect of different absorption enhancers was also investigated．The results indicated that sodium tau