铝诱导柱花草和黑麦根系分泌有机酸及其调控机理的研究-植物营养学专业毕业论文.docxVIP

广西大学硕士学位论文心通道抑制剂CsCl2、四乙基氯化铵(TEACI)对有机酸的分泌无 广西大学硕士学位论文 心通道抑制剂CsCl2、四乙基氯化铵(TEACI)对有机酸的分泌无 显著抑制作用； 250、500 btmol L1的Ca2+专一性螯合剂EGTA显著 抑制柠檬酸的分泌，而对苹果酸分泌无显著影响。这些结果暗示， 阴离子通道参与铝诱导的黑麦根系分泌有机酸，SA和Ca2+可能介 导此分泌过程，而IC对有机酸的分泌无直接调控作用。 关键词：柱花草黑麦铝忍耐有机酸分泌调控阴离子通道 广西大学硕士学位论文A 广西大学硕士学位论文 A STUDY oN Al-INDUCED SECRETIoN oF 0RGANIC ACIDS FROM ROoTS oF BoTH STYLo AND RYE AND CoRRESPoND REGULAl’IoN MECHANlSM ABSTRACT As an important forage legume，Stylo(Stflosanthes spp)has been widely planted in acid soil areas．However,there is still no study on aluminum-tolerance of stylo and its tolerance mechanism at present． The present thesis showed that root elongation of alfalfa(Medicago sativa L．)during a 24一h period was inhibited by 80％by the exposure to 5 umol L。1 A1 and its roots WaS obviously stained by eriocllrome cyanine R(ER)；however,the roots of stylo need to be exposure to 30I-tmol LJ Al in order to get obviously stained with its root elongation was inhibited by 68％，which shows，for stylo，its ability of tolerance to aluminum is strong．According to analysis of root exudates by HPLC， aluminum induced roots of stylo to secret citrate and its secretion increased with A1 concentration treated(O，10，20，50 gmol L‘。)and the period of exposure to A1(6，1 2，1 8，24 h)，which indicated that AI-induced secretion of citrate is an important mechanism for stylo to resist the toxicity of aluminum． {i{ 广西大学硕士学位论文On 广西大学硕士学位论文 On the other hand，although in recent years more and more researches concluded that AI-induced secretion of organic acids from roots is a main mechanism for some A1一tolerance plants，SO far in field of study the knowledge of the mechanism responsible for AI—induced secretion of organic acids is still very limited．In present study，taking rye(Secale cereale L．)which can secreted citrate and malate as study object，I explored the regulation of organic acid secretion from roots Secretion of citrate and malate was obviously inhibited by anion channel inhibitors such as niflumic acid and phen