棉花黄萎病、枯萎病拮抗细菌的筛选及相关抗病基因的克隆-生化与分子生物学专业毕业论文.docxVIP

AbstractCotton Abstract Cotton Verticillium and Fusazium wilts are colnnloB plant di∞as鼯。and hard to be controlled．皿ey callse serious damage to the wodd economy．It is urgent to isolate the antagonistic bacteria and develop the biological pesticides to control the diseases．In this research，wo screened 5 antagonistic bacteria which carl strongly inhibit Verticillium wilt pathogen and Fusarium wnt pathogen．and then cloned the resistance-related gene． 21 bacteria wefe Isolated from COttOn land rhizosphere soil from The PIant Protection Institute of The Chinese Academy of Agricultural Sciences．And 5 of them can strongly inhibit Verticillium wilt pathogen and Fusariwn wilt pathogen．The antibacterial efficiency of ferngntation broth of C5，C6,S6． Sl，BI are about 22．6％，38．7％，53．1％，52．4％，21％(against Verticillium wilt pathogen)。and 22．8％， 38．7％，55．2％，56％，23．5％(against Fusarium wilt pathogen)．The swain S6 and S1 can be develop雏 biological pesticides． All of the 5 antsgonisfic bacteria am positive bacteria．S6 was identified as Bac／／／us sp．by the method of 16s rDNA．S6 grows very fasL It grows well in the temperature between 15℃and 50C．the optimum据棚p叮anl他is28C；it alsogrowswellinthepHbetween4and 9，theoptimumpHis 7． S6 couldn’t resistance to the antibiotic ofjunp，Cm．my．Rif,K札but can weak resistance to the concentration of 25“g／mL of Spc and Sm．It shows that the antibiotic components including the volatile component and the cell wall-degrading enzyme．The activity of the chitinase is 6．04U；the glucanase is 24．86U；the peetinase is 23．37U；the cellul撇is 10．37U． The glucanase is important antifungal protein．With specific primers designed．700bp PCR product wag amplified,the fragment wag sequenced and closed related to glucanase with 69％． Constructing mutation library of S6 by piC333．3 mutations w讹isolated．One of the them wag sequenced，and the sequences a∞closely related to iturin A synthetaso B with 99％． Key words：Verticillium wilt Fusarium wilt Bacillus sp． clone gene II 独创性声明本人声明所呈交