抗生素电化学性质的研究与应用-分析化学专业毕业论文.docxVIP

山东师范人学硕士学位论文抗生素电化学性质的研究与应用 山东师范人学硕士学位论文 抗生素电化学性质的研究与应用 摘要 本论文在B—R缓冲溶液中以静汞电极、滴汞电极为工作电极，用 循环伏安法、线性扫描伏安法、线性扫描二阶导数极谱法等电化学方 法对甲基蓝、苯胺蓝、鸡冠花红等与抗生素类药物相互作用的条件进 行了较为详细的研究，发现这些有机染料在电极上有良好的伏安还原 峰。当加入抗生素后，有机染料分子与抗生素发生作用，使有机染料 的伏安还原峰电流值降低而电位基本不变，还原峰电流的降低值与抗 生素的加入量在一定范围内呈良好的线性关系。实验绘制了测定抗生 素的工作曲线，并成功地将该方法应用于临床抗生素药物含量的测 定，结果与经典的分光光度法一致。 该论文对抗生素与有机染料分子相互作用的机理进行了初步探 讨，计算了抗生素与染料分子相互作用的结合比和结合常数。根据有 机染料分子与抗生素作用前后的紫外可见吸收光谱图初步推断，二者 能够发生作用，并生成新复合物。通过电化学分析方法进一步表明有 机染料分子与抗生素相互作用生成了非电化学活性的超分子复合物， 降低了溶液中有机染料的浓度，从而染料分子的还原峰电流降低。 另外，研究了聚茜素红薄膜修饰电极对司帕沙星的电催化作用， 测定了市售司帕沙星片剂中司帕沙星的含量，从而进一步提出了电化 学分析法测定抗生素的新方法。 关键词：伏安法：有机染料；抗生素；还原峰电流；修饰电极 山东师范大学硕士学位论文Study 山东师范大学硕士学位论文 Study and Application on the Electrochemical Property of Antibiotics ABSTRACT In this paper，in B—R buffer solution，the reaction conditions of the interaction of antibiotics with Methyl blue，aniline blue，amaranth were investigated using static mercury electrode and dropping mercury electrode as working electrode by electrochemical methods such as the cyclic voltammetry，linear scan voltammetry and the second order derivative polarography，et a1．These organic dyes have well—defined voltammetric reductive peak．Adding antibiotics to above solution，the antibiotics interacted with organic dye molecules and made the peak current of organic dye molecules decrease，while the voltage of the reductive peak did not change nearly．The decrease of the peak current was linearly with the concentration of antibiotics ，which may be used to the determination of antibiotics．In this method the regressions curve of antibiotics were worked out and further applied to determinate the antibiotic samples and the results were consistent with the spectrophotometry． The mechanism of the interaction of antibiotics with 4 山东师范大学硕士学位论文organic 山东师范大学硕士学位论文 organic dye molecules was originally discussed，and the binding ratio and binding constant were also calculated．According to the UV—Vis absorption spectra of the reaction system，it was illustrated that organic dye molecules may